Sub-diffraction limit image resolution and other imaging techniques
The present invention generally relates to sub-diffraction limit image resolution and other imaging techniques. In one aspect, the invention is directed to determining and/or imaging light from two or more entities separated by a distance less than the diffraction limit of the incident light. For example, the entities may be separated by a distance of less than about 1000 nm, or less than about 300 nm for visible light. In one set of embodiments, the entities may be selectively activatable, i.e., one entity can be activated to produce light, without activating other entities. A first entity may be activated and determined (e.g., by determining light emitted by the entity), then a second entity may be activated and determined. The entities may be immobilized relative to each other and/or to a common entity. The emitted light may be used to determine the positions of the first and second entities, for example, using Gaussian fitting or other mathematical techniques, and in some cases, with sub-diffraction limit resolution. The methods may thus be used, for example, to determine the locations of two or more entities immobilized relative to a common entity, for example, a surface, or a biological entity such as DNA, a protein, a cell, a tissue, etc. The entities may also be determined with respect to time, for example, to determine a time-varying reaction. Other aspects of the invention relate to systems for sub-diffraction limit image resolution, computer programs and techniques for sub-diffraction limit image resolution, methods for promoting sub-diffraction limit image resolution, methods for producing photoswitchable entities, and the like.
1. A system, comprising:
a first light source which applies activation light for activating a part of a plurality of photoswitchable entities into a state able to emit light;
a second light source which applies excitation light for exciting at least a part of the activated entities;
a detector which detects at least a part of light emitted from the excited entities; and
a controller, wherein the controller is programmed to: calculate positional information of at least a part of the plurality of entities by using information contained in the detected light, correct the positional information by using a fiduciary marker and movements of the fiduciary marker.
2. The system of claim 1 , wherein the entities are fluorescent proteins.
3. The system of claim 1 , wherein the entities are cyanine dyes.
4. The system of claim 3 , wherein the cyanine dyes comprise at least one of Cy5, Cy5.5, Cy7, Alexa Fluor® 647, Alexa Fluor® 405, Alexa Fluor® 488, Cy2, Cy3, Cy3.5, Cy5, and a conjugate thereof.
5. The system of claim 1 , wherein the fiduciary marker is a fluorescent particle.
6. The system of claim 1 , wherein the controller is able to use Gaussian fitting to calculate the positional information.
7. The system of claim 1 , wherein the controller is able to use elliptical Gaussian fitting to calculate the positional information.
8. The system of claim 1 , wherein the activation light and the excitation light have substantially the same wavelength.
9. The system of claim 1 , wherein the activation light and the excitation light have different wavelengths.
10. The system of claim 1 , wherein the controller is able to generate an image by using the calculated positional information.
11. The system of claim 10 , wherein the image is a high resolution image.
12. The system of claim 1 , wherein the plurality of entities comprises a first entity and a second entity.
13. The system of claim 12 , wherein:
the first entity is activatable by a first wavelength of activation light and
the second entity is activatable by a second wavelength of activation light.
14. The system of claim 12 , wherein:
the first entity is excitable by a first wavelength of excitation light and
the second entity is excitable by a second wavelength of excitation light.
15. The system of claim 1 , wherein the entities have a first portion and a second portion, the first portion being a light emitting portion and the second portion being an activator portion.
16. The system of claim 1 , wherein the controller is able to calculate positional information of at least some of the plurality of entities to a resolution smaller than 20 nm.
17. The system of claim 1 , wherein the controller is able to use the positional information to construct an image, the image having a resolution that is better than the diffraction-limited resolution of the emitted light.
18. The system of claim 1 , wherein the controller is able to calculate the positional information of the at least a part of the plurality of entities at more than one point of time and/or as a function of time.
19. The system of claim 1 , wherein at least some of the plurality of entities are activatable by light of different wavelengths and/or emit light at different wavelengths.
20. The system of claim 1 , wherein the correction of the positional information comprises drift correction.
21. A program for causing a machine to perform a procedure of calculating positional information performed by a system as claimed in claim 1 .
22. A storage medium comprising a program for causing a machine to perform a procedure of calculating positional information performed by a system as claimed in claim 1 .
23. An article, comprising a storage medium comprising a program for causing a machine to perform a procedure of calculating positional information performed by a system as claimed in claim 1 .
24. A method, comprising:
applying activation light for activating a part of a plurality of photoswitchable entities into a state able to emit light;
applying excitation light for exciting at least a part of the activated entities;
detecting at least a part of light emitted from the excited entities; and
calculating positional information of at least a part of the plurality of entities by using information contained in the detected light, correcting the positional information by using a fiduciary marker and movements of the fiduciary marker.
25. The method of claim 24 , wherein the correction is drift correction.