Smallpox vaccine for cancer treatment
Disclosed herein are methods and compositions related to therapy for cancer. More specifically, the disclosed methods and compositions are related to the use of smallpox vaccine to induce an effective anti-tumor immune response.
1. A method for treating a solid tumor or hematologic malignancy in a subject, comprising administering to the subject an oncolytic virus, and a composition comprising an adipose stromal cell, wherein the oncolytic virus is a vaccinia virus.
2. The method of claim 1 , wherein the adipose stromal cell comprises an artificial chromosome, virus or plasmid.
3. A method for treating a solid tumor or hematologic malignancy in a subject, comprising administering to the subject an oncolytic virus, and a composition comprising an adipose stromal cell, wherein the oncolytic virus is a poxvirus.
4. The method of claim 1 , wherein the oncolytic virus does not comprise heterologous nucleic acid that encodes a heterologous product.
5. The method of claim 1 , wherein the vaccinia virus is an attenuated New York City Board of Health (NYCBOH) strain of vaccinia virus.
6. The method of claim 5 , wherein the NYCBOH strain of vaccinia virus has all of the identifying characteristics of the strain deposited as ATCC VR-118 or CJ-MVB-SPX.
7. The method of claim 1 , wherein the vaccinia virus is selected from among Dryvax, ACAM1000, ACAM2000, Lister, EM63, LIVP, Tian Tan, Copenhagen, Western Reserve, Modified Vaccinia Ankara (MVA), New York City Board of Health, Dairen, Ikeda, LC16M8, Western Reserve Copenhagen, Tashkent, Tian Tan, Wyeth, IHD-J, and IHD-W, Brighton, Dairen I and Connaught strains.
8. The method of claim 1 , wherein the solid tumor is glioblastoma, breast carcinoma, lung carcinoma, prostate carcinoma, colon carcinoma, ovarian carcinoma, neuroblastoma, central nervous system tumor, or melanoma.
9. The method of claim 1 , wherein the oncolytic virus encodes a heterologous gene product.
10. The method of claim 1 , wherein the subject is human.
11. The method of claim 10 , wherein the subject is a pediatric patient.
12. The method of claim 1 , wherein the composition comprising an adipose stromal cell comprises an adipose-derived stromal vascular fraction (SVF).
13. The method of claim 12 , wherein the adipose-derived SVF is autologous.
14. The method of claim 1 wherein the virus and the adipose stromal cell are cultured together in vitro prior to administration to the subject.
15. A composition comprising:
(a) smallpox vaccine; and
(b) an adipose-derived stromal vascular fraction (SVF).
16. The method of claim 1 , wherein the oncolytic virus is a smallpox vaccine.
17. The method of claim 12 , wherein the oncolytic virus is ACAM2000.
18. The method of claim 13 , wherein the oncolytic virus is ACAM2000.
19. The method of claim 1 , wherein the vaccinia virus is ACAM2000.
20. The composition of claim 15 , wherein the smallpox vaccine is Dryvax, ACAM2000 or ACAM 1000.
21. The method of claim 1 , wherein the virus is administered by intratumoral, intravenous, intraperitoneal, intrathecal, intraventricular, intraarticular, or intraocular injection.
22. The method of claim 1 , wherein the adipose stromal cell is administered by intratumoral, intravenous, intraperitoneal, intrathecal, intraventricular, intraarticular, or intraocular injection.
23. The method of claim 1 , wherein the virus is mixed with the composition comprising the adipose stromal cell prior to administration.
24. The method of claim 2 , wherein the adipose stromal cell is modified with a virus, plasmid or artificial chromosome.
25. The composition of claim 15 , wherein the smallpox vaccine is ACAM2000.
26. The composition of claim 15 , wherein the smallpox vaccine is an attenuated strain selected from among Dryvax, ACAM1000, ACAM2000, Lister, EM63, LIVP, Tian Tan, Copenhagen, Western Reserve, Modified Vaccinia Ankara (MVA), New York City Board of Health, Dairen, Ikeda, LC16M8, Western Reserve Copenhagen, Tashkent, Tian Tan, Wyeth, IHD-J, and IHD-W, Brighton, Dairen I and Connaught strains.
27. The method of claim 1 , wherein the tumor is a solid tumor.
28. The method of claim 7 , wherein:
the tumor is a solid tumor; and
the virus is ACAM 2000.