Acidic biofilm remediation
Methods and compositions for biofilm remediation are disclosed. Biofilm is reduced and removed from soiled surfaces by providing to a surface in need an effective amount of a composition comprising a biocidal surfactant and at least one organic solvent. According to the invention, the biofilm remediation compositions reduce and remove biofilm formation by administering a one-step cleaner and disinfectant. The biofilm remediation compositions are stable and effective in concentrated and diluted ready-to-use formulations comprising an anionic sulfated or sulfonated surfactant that is not an organocarboxylic acid, a sparingly soluble organic solvent and optionally a soluble organic solvent.
1. A concentrated composition comprising:
from about 1 to about 30 wt-% of an anionic surfactant, wherein the anionic surfactant excludes short chain (C1-C5) organocarboxylic acid and is a sulfonated acid;
from about 5 to about 25 wt-% of a soluble organic solvent; and
from about 10 to about 40 wt-% of a sparingly soluble organic solvent;
wherein the ratio of said soluble organic solvent to said sparingly soluble organic solvent is between about 10:1 and about 1:10 by weight percent;
wherein said sparingly soluble organic solvent is soluble in the soluble organic solvent to an extent of less than about 20 wt-%;
wherein said composition has sanitizing efficacy against a biofilm by penetrating the biofilm's extracellular matrix comprising polysaccharides; and
wherein the pH of a diluted solution produced from the concentrated composition is less than about 3;
wherein the composition provides a 4-log order reduction of microbes in a biofilm-soiled surface; and
wherein the microbes comprise one or more of a Pseudomonas , an Escherichia , a Staphylococcus , and/or a Listeria.
2. The composition of claim 1 wherein the anionic surfactant is selected from the group consisting of (C 8 -C 22 ) alkylsulfonate, linear alkyl benzene sulfonic acid, diphenylated sulfonates, sulfonated oleic acid, and mixtures thereof.
3. The composition of claim 1 wherein said sparingly soluble organic solvent is a glycol ether selected from the group consisting of diethylene glycol n-butyl ether, diethylene glycol n-propyl ether, diethylene glycol ethyl ether, diethylene glycol methyl ether, diethylene glycol t-butyl ether, dipropylene glycol n-butyl ether, dipropylene glycol methyl ether, dipropylene glycol ethyl ether, dipropylene glycol propyl ether, dipropylene glycol tert-butyl ether, ethylene glycol butyl ether, ethylene glycol propyl ether, ethylene glycol ethyl ether, ethylene glycol methyl ether, ethylene glycol methyl ether acetate, propylene glycol n-butyl ether, propylene glycol ethyl ether, propylene glycol methyl ether, propylene glycol n-propyl ether, tripropylene glycol methyl ether and tripropylene glycol n-butyl ether, ethylene glycol phenyl ether, propylene glycol phenyl ether and mixtures thereof.
4. The composition of claim 3 , wherein the sparingly soluble and soluble organic solvents are a mixture of dipropylene glycol methyl ether and propylene glycol phenyl ether.
5. The composition of claim 4 wherein the composition comprises between about 5 and 25 wt-% of dipropylene glycol methyl ether and between about 10 and 40 wt-% of propylene glycol phenyl ether.
6. The composition of claim 1 further comprising between about 0.01 and about 35 wt. % of an oxidizing agent, wherein the oxidizing agent comprises hydrogen peroxide having an active concentration of 50% or greater; and wherein said anionic surfactant is from about 5 to about 25 wt-%.
7. The composition of claim 6 , wherein hydrogen peroxide has an active concentration of 70% or greater; and wherein the composition does not include a peroxycarboxylic acid or a halogen.
8. The composition of claim 7 wherein the anionic surfactant is selected from the group consisting of (C 8 -C 22 ) alkylsulfonate, linear alkyl benzene sulfonic acid, diphenylated sulfonates, sulfonated oleic acid, and mixtures thereof and wherein the sparingly soluble and soluble organic solvents are selected from the group consisting of diethylene glycol n-butyl ether, diethylene glycol n-propyl ether, diethylene glycol ethyl ether, diethylene glycol methyl ether, diethylene glycol t-butyl ether, dipropylene glycol n-butyl ether, dipropylene glycol methyl ether, dipropylene glycol ethyl ether, dipropylene glycol propyl ether, dipropylene glycol tert-butyl ether, ethylene glycol butyl ether, ethylene glycol propyl ether, ethylene glycol ethyl ether, ethylene glycol methyl ether, ethylene glycol methyl ether acetate, propylene glycol n-butyl ether, propylene glycol ethyl ether, propylene glycol methyl ether, propylene glycol n-propyl ether, tripropylene glycol methyl ether and tripropylene glycol n-butyl ether, ethylene glycol phenyl ether, propylene glycol phenyl ether and mixtures thereof.
9. The composition of claim 8 wherein soluble organic solvent and the sparingly soluble organic solvents are dipropylene glycol methyl ether and propylene glycol phenyl ether.
10. The composition of claim 1 wherein the composition is diluted with water to form a diluted solution, wherein the dilution is between about 5 parts water per 1 part concentrated composition and about 128 parts water per 1 part concentrated composition.
11. The composition of claim 1 wherein the composition is diluted with water to form a diluted solution, wherein the dilution is between about 16 parts water per 1 part concentrated composition and about 64 parts water per 1 part concentrated composition.
12. The composition of claim 11 wherein the composition is diluted with water to form a diluted solution, wherein the dilution is less than about 32 parts water per 1 part concentrated composition.
13. A biocidal composition comprising:
from about 0.1 to about 2 wt-% of an anionic surfactant, wherein the anionic surfactant is not a short chain (C1-C5) organocarboxylic acid and is a sulfonated acid;
from about 0.1 to about 2 wt-% of a soluble organic solvent in dilution; and
from about 0.5 to about 5 wt-% of a sparingly soluble organic solvent in dilution;
wherein the ratio of said soluble organic solvent to said sparingly soluble organic solvent is between about 10:1 and about 1:10 by weight percent;
wherein said sparingly soluble organic solvent is soluble in the soluble organic solvent to an extent of about 15 wt-% or less;
wherein the composition has a pH of less than about 3;
wherein the composition provides a 4-log order reduction of microbes in a biofilm-soiled surface; and
wherein the microbes comprise one or more of a Pseudomonas aeruginosa , a Staphylococcus epidermidis , a Staphylococcus aureus , a Listeria monocytogenes , and/or an Escherichia coli.
14. The composition of claim 13 wherein the anionic surfactant is selected from the group consisting of (C 8 -C 22 ) alkylsulfonate, linear alkyl benzene sulfonic acid, diphenylated sulfonates, sulfonated oleic acid, and mixtures thereof.
15. The composition of claim 13 wherein the sparingly soluble organic solvent is selected from the group consisting of diethylene glycol n-butyl ether, diethylene glycol n-propyl ether, diethylene glycol ethyl ether, diethylene glycol methyl ether, diethylene glycol t-butyl ether, dipropylene glycol n-butyl ether, dipropylene glycol methyl ether, dipropylene glycol ethyl ether, dipropylene glycol propyl ether, dipropylene glycol tert-butyl ether, ethylene glycol butyl ether, ethylene glycol propyl ether, ethylene glycol ethyl ether, ethylene glycol methyl ether, ethylene glycol methyl ether acetate, propylene glycol n-butyl ether, propylene glycol ethyl ether, propylene glycol methyl ether, propylene glycol n-propyl ether, tripropylene glycol methyl ether and tripropylene glycol n-butyl ether, ethylene glycol phenyl ether, propylene glycol phenyl ether and mixtures thereof.
16. The composition of claim 15 wherein the sparingly soluble and soluble organic solvents are dipropylene glycol methyl ether and propylene glycol phenyl ether.
17. The composition of claim 13 , further comprising between about 0.01 and about 35 wt. % of an oxidizing agent, wherein the oxidizing agent comprises hydrogen peroxide having an active concentration of 70% or greater, and wherein the composition does not contain a peroxycarboxylic acid or a halogen.
18. A method of biofilm remediation comprising:
contacting a biofilm with the biocidal composition of claim 13 ; wherein the biocidal composition penetrates the biofilm's extracellular matrix comprising polysaccharides.
19. The method of claim 18 , wherein the biofilm is reduced by at least three log CFU/cm 2 after ten minutes of exposure to the composition.
20. The method of claim 18 , wherein the anionic surfactant is selected from the group consisting of (C 8 -C 22 ) alkylsulfonate, linear alkyl benzene sulfonic acid, diphenylated sulfonates, sulfonated oleic acid, and mixtures thereof, and wherein the sparingly soluble organic solvent is selected from the group consisting of diethylene glycol n-butyl ether, diethylene glycol n-propyl ether, diethylene glycol ethyl ether, diethylene glycol methyl ether, diethylene glycol t-butyl ether, dipropylene glycol n-butyl ether, dipropylene glycol methyl ether, dipropylene glycol ethyl ether, dipropylene glycol propyl ether, dipropylene glycol tert-butyl ether, ethylene glycol butyl ether, ethylene glycol propyl ether, ethylene glycol ethyl ether, ethylene glycol methyl ether, ethylene glycol methyl ether acetate, propylene glycol n-butyl ether, propylene glycol ethyl ether, propylene glycol methyl ether, propylene glycol n-propyl ether, tripropylene glycol methyl ether and tripropylene glycol n-butyl ether, ethylene glycol phenyl ether, propylene glycol phenyl ether and mixtures thereof.