Agent for the treatment and or prophylaxis of an autoimmune disease and for the formation of regulatory T cells
The present invention relates to an agent for the treatment and/or prophylaxis of an autoimmune disease, an agent for the formation of regulatory T cells (T Reg ) in an organism and various methods in which the agents according to the invention are used.
1. A method for the treatment of an autoimmune disease in an organism, the method comprising:
(a) contacting peripheral mononuclear blood cells (PBMCs) derived from a first organism with a mutein of human interleukin-2 (hIL-2 mutein), wherein said hIL-2 mutein has an amino acid substitution in at least one of the positions 20, 88, or 126, numbered in accordance with the hIL-2 wild type sequence as set forth in SEQ ID NO: 1, to obtain a cell population which comprises regulatory T cells, and
(b) introducing the cell population into a second organism for the treatment of the autoimmune disease in the second organism, wherein the autoimmune disease is selected from the group consisting of type I diabetes, multiple sclerosis, and systemic lupus erythematosus (SLE).
2. The method of claim 1 , wherein the first and the second organisms are the same individual or are individuals of the same species.
3. The method of claim 1 , wherein in said hIL-2 mutein, through the substitution at position 88, an asparagine is exchanged for an amino acid which is selected from the group consisting of: arginine (hIL-2-N88R), glycine (hIL-2-N88G), or isoleucine (hIL-2-N88I).
4. The method of claim 1 , wherein said hIL-2 mutein has at least one further amino acid substitution in any position except the positions 20, 88, or 126, and wherein the at least one further substitution is a conservative amino acid substitution.
5. The method of claim 1 , wherein in said hIL-2 mutein, through the substitution at position 20, an aspartic acid is exchanged for an amino acid which is selected from the group consisting of: histidine (hIL-2-D20H), isoleucine (hIL-2-D200, or tyrosine (hIL-2-D20Y).
6. The method of claim 1 , wherein in said hIL-2 mutein, through the substitution at position 126, a glutamine is exchanged for a leucine (hIL-2-Q126L).
7. The method of claim 1 , wherein the method further comprises administering to the second organism an immunosuppressant.
8. The method of claim 7 , wherein the immunosuppressant is selected from the group consisting of: glucocorticoid, including decortin, prednisol; azathioprine; cyclosporin A; tacrolimus; an anti-T lymphocyte globulin; an anti-CD3 antibody; muromonab; an anti-CD25 antibody; basiliximab; daclizumab; an anti-TNF-α antibody; infliximab; adalimumab; azathioprine; methotrexate; cyclosporin; sirolimus; everolimus; fingolimod; CELLCEPT® (mycophenolate mofetil); myfortic; and cyclophosphamide.