Method of reducing scar formation in healing of dermal wounds by administering interleukin-10 and hyaluronan
A sustained release formulation of interleukin-10 for wound treatment and related methods is provided.
1. A method for reducing scarring by administering a therapeutically effective amount of IL-10 to an incision or wound site in a subject for a time sufficient or reduce scarring, said administering comprising administration of a composition to the incision or wound site in the subject;
wherein the composition is an extended release biocompatible composition for reducing scarring of an incision or wound site in the subject, wherein the composition comprises a biodegradable support scaffold and IL-10, wherein the biodegradable support scaffold is at least one of hyaluronan, a hyaluronan moiety, heparin, heparan sulfate, collagen, PEG, a PEG moiety or a cross-linker and wherein the amount of IL-10 in the composition is from 0.2 μM to 2.5 μM.
2. A method for reducing scarring comprising administering a therapeutically effective amount of IL-10 to an incision or wound site in a subject for a time sufficient to reduce scarring, said administering comprising administration of a composition to a site at risk for scarring in the subject;
wherein the composition is an extended release biocompatible composition for reducing scarring of an incision or wound site in a subject, wherein the composition comprises a biodegradable support scaffold and IL-10, wherein the biodegradable support scaffold is at least one of hyaluronan, a hyaluronan moiety, heparin, heparan sulfate, collagen, PEG, a PEG moiety or a cross-linker and wherein the amount of IL-10 in the composition is from 0.2 μM to 2.5 μM.
3. A method for reducing scarring in a subject, comprising:
providing a composition, wherein the composition is an extended release biocompatible composition for reducing scarring of an incision or wound site in a subject, wherein the composition comprises a biodegradable support scaffold and IL-10, wherein the biodegradable support scaffold is at least one of hyaluronan, a hyaluronan moiety, heparin, heparan sulfate, collagen, PEG, a PEG moiety or a cross-linker and wherein the amount of IL-10 in the composition is from 0.2 μM to 2.5 μM;
introducing the composition to an incision or wound site by administration of the composition; and
maintaining an amount of IL-10 for a time sufficient or reduce scarring, wherein the time sufficient to reduce scarring is at least 1 day, and wherein the amount of IL-10 released is 0.15 nM to 0.150 μM.
4. The method of claim 1 , wherein the administration is a single administration of the composition.
5. The method of claim 1 , wherein the time sufficient to reduce scarring is at least 1 day.
6. The method of claim 1 , wherein the amount of IL-10 released is 0.15 nM to 0.15 μM.
7. The method of claim 2 , wherein the site is a dermal layer or epidermis.
8. The method of claim 1 , wherein the biodegradable support scaffold is configured to release from 0.015 nM to 0.015 μM of IL-10 at the incision or wound site.
9. The method of claim 1 , wherein the biodegradable support scaffold comprises hyaluronan or a hyaluronan moiety.
10. The method of claim 9 , wherein the hyaluronan is hyaluronic acid.
11. The method of claim 1 , wherein the biodegradable support scaffold comprises a heparin or heparan sulfate.
12. The method of claim 1 , wherein the biodegradable support scaffold comprises collagen.
13. The method of claim 12 , wherein the collagen is human collagen.
14. The method of claim 13 , wherein the collagen is Type-I collagen.
15. The method of claim 1 , wherein the biodegradable support scaffold comprises PEG or a PEG moiety.
16. The method of claim 1 , wherein the amount of IL-10 in the composition is from 0.017 μM to 0.035 μM.
17. The method of claim 9 , wherein the biodegradable support scaffold further comprises heparin or heparan sulfate.
18. The method of claim 1 , wherein the scarring is a keloid.