DNA chip with micro-channel for DNA analysis
Provided is a DNA chip with micro-channel for DNA analysis, which has a structure in which a silicon layer (chip A) and a plastic layer (chip B) are laminated, wherein the chip A includes at least two PCR reactors connected in series in a micro-channel, and a filter between the PCR reactors, the chip B includes a reagent, a liquid delivery mechanism and a sensor in a micro-channel, and the reagent, liquid delivery mechanism and sensor can be changed according to a kind of an analyte and an object to be detected.
1. A DNA chip with micro-channel for DNA analysis of DNA included in an analyte according to PCR method, the DNA chip comprising:
a first layer made of silicon; and
a second layer made of plastic,
wherein the second layer is formed on the first layer, and the second layer is configured to be changeably selected depending on a kind of the analyte and DNA, the first layer includes:
at least four openings;
two reactors connected in series,
wherein a first reactor is configured to extract nucleic acids from a sample in the first reactor and a second reactor configured to selectively amplify DNA,
wherein each reactor has a thermocycler that increases and decreases the temperature of each reactor, and
wherein each reactor has a peripheral area made of silicon around each reactor, the peripheral area having a hollowed out shape except for an area connected to the micro-channel;
at least one filter provided between the first reactor and the second reactor,
wherein the filter comprises a plurality of column pillars made of silicon formed by etching, a space between column pillars ranging from 1 micro-meters to 10 micro-meters, and the filter separates and removes blood-derived blood cells; and
a micro-channel connecting among the openings, the first reactor, the second reactor and the filter,
the second layer includes:
a pump; and
a sensor,
wherein a reagent covers at least one opening included in the four openings when one sees from normal direction of the first layer,
the pump is located over at least two openings included in the four openings when one sees from normal direction of the first layer,
the pump supplies the reagent to the reactors via the micro-channel such that mixture of the reagent and the analyte is supplied to the reactors,
the mixture is transported to the sensor via at least one opening included in the four openings such that the sensor analyzes DNA included in the analyte.
2. The DNA chip according to claim 1 , wherein a polymer actuator is used as a pump.
3. DNA analysis method of DNA included in an analyte according to PCR method by using a DNA chip with a micro-channel, the method comprising:
(a′) providing the DNA chip of claim 1
wherein a reagent covers at least one opening included in the four openings when one sees from normal direction of the first layer,
the pump is located over at least two openings included in the four openings when one sees from normal direction of the first layer,
(d) supplying the analyte to the reactors;
(e) supplying the reagent to the PCR reactors via the micro-channel by using the pump such that mixtures of the reagent and the analyte are supplied to the PCR reactors;
(f) performing PCR method to obtain PCR products;
(g) transporting the PCR products to the sensor via at least one opening included in the four openings; and
(h) detecting the PCR products by using the sensor to analyze DNA included in the analyte.