Composition comprising plant-derived recombinant human serum albumin, lipids, and plant protein hydrolysates as active ingredients for cryopreservation of stem cells or primary cells
The present invention relates to a composition including plant-derived recombinant human serum albumin, lipids, and plant protein hydrolysates as active ingredients for cryopreservation of stem cells or primary cells, and use thereof, more particularly, to a composition including plant-derived recombinant human serum albumin, lipids, and plant protein hydrolysates as active ingredients for cryopreservation of stem cells or primary cells, in which the albumin, lipids, and plant protein hydrolysates are present in sufficient quantities to reduce cell death caused by freezing while maintaining animal-free and xeno-free conditions during long-term preservation of stem cells or primary cells; and to a method for cryopreservation of stem cells or primary cells using the composition.
1. A composition for cryopreservation of stem cells or primary cells, comprising plant-derived recombinant human serum albumin, lipids, plant protein hydrolysate, and a cryoprotectant as active ingredients, wherein the stem cells are bone marrow mesenchymal stem cells or umbilical cord mesenchymal stem cells, the primary cells are fibroblasts, the cryoprotectant is selected from the group consisting of dimethyl sulfoxide, glycerol, propylene glycol, ethylene glycol, sucrose, carboxymethylcellulose salts, and carboxymethylcellulose, and the composition contains 40 parts by weight of plant-derived recombinant human serum albumin, 0.1 part by weight to 1 part by weight of lipids, and 1 part by weight to 10 parts by weight of plant protein hydrolysate, based on 100 parts by weight of the total composition.
2. The composition of claim 1 , wherein the lipids contain cholesterol.
3. The composition of claim 1 , further comprising a buffering agent or an isotonic agent.
4. A method for cryopreservation of stem cells or primary cells, comprising freezing stem cells or primary cells which are treated with a composition of claim 1 , wherein the stem cells are bone marrow mesenchymal stem cells or umbilical cord mesenchymal stem cells and the primary cells are fibroblasts.
5. The method of claim 4 , wherein the lipids contain cholesterol.
6. The method of claim 4 , wherein the composition further comprises a buffering agent or an isotonic agent.