Device and method for chemical, biochemical, biological and physical analysis, re-action, assay and more
The invention is directed to a device and a method for carrying out experiments of chemical, biochemical, biological and physical analysis, reaction and assay employing a reaction unit, in a preferred embodiment comprising: a capillarity reaction chamber, non-capillarity zone and bottom structure, which can use capillary action to quantitatively take up by itself and hold liquid in the reaction chamber. A multi-unit plate, which contains a number of reaction units on a plate frame, can be used for parallel experiments in conjunction with reservoir plate, waster pad, and liquid transfer guider.
1. A reaction unit comprising:
a tubular first chamber having:
a first interior space which is a capillary and has a first cross-section having a first diameter,
wherein the first interior space is configured so that a liquid fills the interior space fully with a fully quantitative amount of said liquid by capillary force; and
a second chamber having:
a second interior space of cylindrical shape whose second cross-section has a second diameter which is larger than the first diameter and is non-capillary,
wherein the first chamber and the second chamber are coupled or integrally formed to create the reaction unit via a third area having an interior space, wherein the first interior space of the first chamber is separated from the interior space of the third area by a well-defined edge,
wherein the interior space of the third area has a conical shape or dome shape widening from the first interior space to the second interior space, is a non-capillary area and limits the liquid in the first chamber to said fully quantitative amount, and
wherein the reaction unit is configured to receive liquid at the first chamber and carry the liquid toward the second chamber against gravity.
2. The reaction unit according to claim 1 , wherein the first chamber and the second chamber are arranged coaxially to each other or at an angle to each other.
3. The reaction unit according to claim 1 , wherein the first interior space of the first chamber has a shape of a cylinder, cone or parabola, square, is rectangular, triangular, circular, oval, has a star-like cross-section or a combination thereof.
4. The reaction unit according to claim 1 , wherein the reaction unit has an open top structure for allowing air to pass through while liquid transfers into or from the first chamber.
5. The reaction unit according to claim 1 , wherein the reaction unit has an open bottom structure through which liquid can flow into or out of the first chamber under said capillary action.
6. The reaction unit according to claim 4 , wherein the reaction unit has an open bottom structure through which liquid can flow into or out of the first chamber under the capillary action.
7. The reaction unit according to claim 1 , wherein the diameter of the first cross-section is 0.01 mm to 3 mm.
8. The reaction unit according to claim 1 , wherein the third area is dome shaped.
9. A multi-unit plate comprising a plurality of reaction units being incorporated into or attached to a plate body, wherein each of the reaction units is a reaction unit according to claim 1 .
10. The multi-unit plate according to claim 9 , wherein the first chambers have different sizes suitable to take up different volumes of liquid.
11. The reaction unit according to claim 1 , wherein the first chamber is an open capillary chamber having a lateral gap.
12. The reaction unit of claim 1 , wherein the reaction unit is integrally formed.
13. The reaction unit of claim 3 , wherein the shape is that of a cylinder.