Human antibody specific to c-Met and preparation method thereof
Provided are an antibody specifically bound to c-Met (c-Met-specific antibody) or fragments thereof, and more specifically, a fully human antibody having high affinity and specificity to c-Met isolated from human Fv(ScFv) phage library, a polynucleotide encoding the antibody, a recombinant vector containing the polynucleotide, a cell transformed with the recombinant vector, and a method of producing the c-Met-specific antibody by culturing the cell, wherein the c-Met-specific antibody may effectively control activity of c-Met in vivo.
1. A c-Met-specific antibody or an antigen binding fragment thereof comprising:
a heavy chain variable region CDR1 having the amino acid sequence of SEQ ID NO: 1; a heavy chain variable region CDR2 having the amino acid sequence of SEQ ID NO: 2; and a heavy chain variable region CDR3 having the amino acid sequence SEQ ID NO: 3; and
a light chain variable region CDR1 having the amino acid sequence of SEQ ID NO: 4; a light chain variable region CDR2 having the amino acid sequence of SEQ. ID NO: 5; and a light chain variable region CDR3 having the amino acid sequence of SEQ ID NO: 6.
2. The c-Met-specific antibody or the antigen binding fragment thereof according to claim 1 , wherein the heavy chain variable region has the amino acid sequence of SEQ ID NO:7.
3. The c-Met-specific antibody or the antigen binding fragment thereof according to claim 1 , wherein the light chain variable region has the amino acid sequence of SEQ ID NO:8.
4. A polynucleotide encoding the c-Met-specific antibody or the antigen binding fragment thereof according to any one of claims 1 to 3 .
5. The polynucleotide according to claim 4 , comprising the nucleotide sequence of SEQ ID NO: 11 or SEQ ID NO: 12.
6. A recombinant expression vector comprising the polynucleotide encoding the c-Met-specific antibody or the antigen binding fragment thereof according to claim 4 .
7. A host cell transformed with the recombinant expression vector according to claim 6 .
8. A method of producing a c-Met-specific antibody or an antigen binding fragment thereof according to claim 1 comprising: culturing the host cell according to claim 7 , and recovering the c-Met-specific antibody or the antigen binding fragment thereof from the culture.