Drought-tolerance in plants
Drought-inducible plant promoters are described herein that are useful for expressing drought tolerance factors in plants.
1. A plant comprising a promoter comprising SEQ ID NO: 11, wherein said promoter is operably linked to a heterologous nucleic acid.
2. The plant of claim 1 , wherein the heterologous nucleic acid comprises at least one coding region.
3. The plant of claim 1 , wherein the heterologous nucleic acid comprises at least one translational initiation region, at least one multi-cloning site, at least one plant transcriptional termination sequence, at least one plant translational termination sequence, or any combination thereof.
4. The plant of claim 1 , wherein the plant comprises an expression cassette, or an expression vector, comprising the nucleic acid segment operably linked to the heterologous nucleic acid.
5. The plant of claim 1 , wherein the heterologous nucleic acid comprises a coding region that encodes a drought tolerance factor.
6. The plant of claim 5 , wherein the drought tolerance factor comprises a sequence with at least 95% amino acid sequence identity to a sequence selected from the group consisting of SEQ ID NO: 14, 16, and 18.
7. The plant of claim 5 , wherein the drought tolerance factor comprises a coding region that has at least 95% nucleotide sequence identity to a sequence selected from the group consisting of SEQ ID NO: 13, 15, and 17.
8. The plant of claim 5 , wherein the heterologous nucleic acid comprises at least one coding region that encodes a drought tolerance factor with at least 95% amino acid sequence identity to SEQ ID NO: 16, and wherein the plant loses over 8 hours of drought conditions at least about 5% less water than a plant that does not have the heterologous nucleic acid that encodes a drought tolerance factor.
9. The plant of claim 1 , wherein the plant is a monocot, dicot, or gymnosperm.
10. The plant of claim 1 , wherein the plant is a plant crop species.
11. A plant seed, a plant cell or plant tissue comprising a promoter comprising SEQ ID NO: 11, wherein said promoter operably linked to a heterologous nucleic acid.
12. The plant seed, a plant cell or plant tissue of claim 11 , wherein the heterologous nucleic acid comprises a coding region that encodes a drought tolerance factor.
13. The plant seed, a plant cell or plant tissue of claim 12 , wherein the drought tolerance factor comprising at least 95% amino acid sequence identity to a sequence selected from the group consisting of SEQ ID NO: 14, 16, and 18.
14. The plant seed, a plant cell or plant tissue of claim 12 , wherein the drought tolerance factor comprises a coding region has at least 95% nucleotide sequence identity to a sequence selected from the group consisting of SEQ ID NO: 13, 15, and 17.
15. A method of producing a drought tolerant plant, the method comprising expressing in a plant a construct comprising nucleic acid encoding a drought tolerance factor operably linked to a heterologous a promoter comprising SEQ ID NO: 11.
16. The method of claim 15 , further comprising (a) transforming one or more plant cells with the construct, and (b) generating a plant from the one or more plant cells.
17. The method of claim 15 , wherein the drought tolerance factor comprises at least 95% amino acid sequence identity to a sequence selected from the group consisting of SEQ ID NO: 14, 16, and 18.
18. The method of claim 15 , wherein the drought tolerance factor comprises a coding sequence comprising at least 95% nucleotide sequence identity to a sequence selected from the group consisting of SEQ ID NO: 13, 15, and 17.
19. The method of claim 15 , wherein the plant is a monocot, dicot, or gymnosperm.
20. The method of claim 15 , wherein the plant is a crop species.
21. The method of claim 15 , wherein the plant is a food crop species, a legume species, a fiber-containing plant species, a tree species, flax, a grain species, a grass species, a woody plant species, a softwood, a hardwood, an oil producing plant species, a starch producing plant species, or a forage plant species.
22. The method of claim 15 , wherein the drought tolerance factor comprises at least 95% amino acid sequence identity to SEQ ID NO: 16, and wherein the plant loses over 8 hours of drought conditions at least about 5% less water than a plant that does not comprise the construct.