Methods for identifying plants with extended shelf life
The invention provides compositions and methods related to selective inhibition of PPO11 and use for improving shelf life of a plant or parts thereof. In accordance with the invention, for example, compositions for topical application to a plant or part thereof, are provided that can reduce browning of the plant or part thereof to extend shelf life.
1. A method for identifying plants having reduced expression or lacking expression of a PPO11 gene, comprising:
a) obtaining a plurality of plants;
b) screening the plurality of plants for expression of a PPO11 gene having at least 95% sequence identity to SEQ ID NO:9 in which expression is positively correlated with browning or reduced shelf life of a processed product of the plant;
c) selecting one or more screened plants comprising decreased expression of the PPO11 gene relative to expression of the PPO11 gene in a reference plant of the same crop species, a parent plant, or an otherwise isogenic plant, and which displays browning or reduced shelf life.
2. The method of claim 1 , wherein the plurality of plants are obtained by random mutagenesis to obtain plants having reduced expression or lacking expression of the PPO11 gene.
3. The method of claim 1 , wherein the plurality of plants are transgenic plants.
4. The method of claim 1 , wherein the plurality of plants comprise 10, 100, or 1000 or more plants.
5. The method of claim 1 , wherein the plurality of plants are varieties of the same species of plants.
6. The method of claim 1 , wherein screening for expression of the PPO11 gene comprises determining an abundance of PPO11 RNA, determining PPO11 activity, or determining abundance of a protein or RNA encoded by SEQ ID NO:9.
7. The method of claim 1 , further comprising crossing the one or more plants with reduced expression of PPO11 to a different plant.
8. A method of lettuce breeding comprising:
a) identifying a first lettuce plant according to the method of claim 1 ; and
b) crossing the first lettuce plant with itself or a second lettuce plant to produce progeny lettuce plants having reduced expression or lacking expression of the PPO11 gene.
9. The method of claim 1 , wherein said plants are lettuce plants.