Irradiation of red blood cells and anaerobic storage
A blood storage system comprising: a collection vessel for red blood cells; an oxygen or oxygen and carbon dioxide depletion device; a storage vessel for red blood cells; tubing connecting the collection vessel to the oxygen or oxygen and carbon dioxide depletion device and the oxygen or oxygen and carbon dioxide depletion device to the storage vessel; and a gamma or X-ray irradiating device is used to irradiate red blood cells stored in the vessel, storing red blood cells under anaerobic conditions.
1. A blood storage system for reducing irradiation damage to red blood cells comprising:
a collection vessel for red blood cells;
an oxygen or oxygen and carbon dioxide depletion device comprising a cartridge; a plurality of hollow fibers or gas-permeable films extending within the cartridge from an entrance to an exit thereof, wherein the hollow fibers or gas-permeable films are adapted to receiving and conveying red blood cells; and an oxygen and carbon dioxide scavenger packed within the cartridge and contiguous to and in between the plurality of hollow fibers or gas-permeable films;
a storage vessel for storing oxygen or oxygen and carbon dioxide-depleted red blood cells under an anaerobic condition;
a first tubing connecting the collection vessel to the oxygen or oxygen and carbon dioxide depletion device; and
a second tubing connecting the oxygen or oxygen and carbon dioxide depletion device to the storage vessel;
wherein said oxygen or oxygen and carbon dioxide-depleted red blood cells are capable of being irradiated with a gamma- or X-ray irradiating device, when stored in said storage vessel; and
wherein said irradiation damage to said oxygen and carbon dioxide-depleted red blood cells is reduced without the addition of L-carnitine or an alkanoul derivative to said blood storage system and said oxygen and carbon dioxide-depleted red blood cells.
2. The blood storage system according to claim 1 , wherein said anaerobic condition is measured as an oxygen-saturation (SO 2 ) of hemoglobin in said red blood cells of less than 20% SO 2 .
3. The blood storage system according to claim 2 , wherein said oxygen-saturation of hemoglobin in said red blood cells is less than 5% SO 2 .
4. The blood storage system according to claim 3 , wherein said oxygen-saturation of hemoglobin in said red blood cells is less than 3% SO 2 .
5. The blood storage system according to claim 1 , wherein said oxygen or oxygen and carbon dioxide depletion device is disposable.
6. The blood storage system according to claim 1 , further comprising a leuko reduction filter disposed between said collection vessel and said oxygen or oxygen and carbon dioxide depletion device.
7. The blood storage system according to claim 1 , further comprising an additive solution vessel in communication with said collection vessel.
8. The blood storage system according to claim 1 , further comprising a plasma vessel in communication with said collection vessel.
9. The blood storage system according to claim 1 , wherein said anaerobic condition is measured as a partial pressure of oxygen (PO 2 ) of less than 0.08 mmHg.
10. The blood storage system according to claim 1 , wherein said storage vessel is substantially impermeable to oxygen or oxygen and carbon dioxide.
11. The blood storage system according to claim 1 , wherein said storage vessel comprises an inner layer compatible with blood and an outer layer substantially impermeable to oxygen or oxygen and carbon dioxide.