IP Library › Granted Patent US 10,144,941

Granted Patent B2

US 10,144,941 · App. 14/851,812 · Granted Dec 4, 2018

Method and compositions for improved lignocellulosic material hydrolysis

Inventors: Brian Grant Fox (Madison, WI); Taichi Takasuka (Madison, WI); Adam Joel Book (Madison, WI); Cameron Robert Currie (Madison, WI)

Assignee: Wisconsin Alumni Research Foundation

C12P19/14 A23K10/12 C12N1/20 C12N9/2434 C12N9/2437 C12N9/2491 C12P19/02 C12Y302/01004 C12Y302/01025 C12Y302/01091

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Quick Facts

Patent No.: US 10,144,941
App. No.: 14/851,812
Granted: Dec 4, 2018
Kind: B2

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Abstract

A method of digesting a lignocellulosic material is disclosed. In one embodiment, the method comprises the step of exposing the material to an effective amount of Streptomyces sp. ActE secretome such that at least partial lignocellulosic digestion occurs.

Claims (111)

1. A microbial host cell comprising at least one exogenous nucleic acid molecule encoding a Streptomyces sp ActE (SActE) enzyme, wherein said enzyme is selected from the group consisting of:

SActE_0237 (GH6),

SActE_0236 (GH48),

SActE_3159 (CMB33),

SActE_0482 (GH5),

SActE_0265 (GH10),

SActE_2347 (GH5),

SActE_0357 (CE4),

SActE_0358 (GH11),

SActE_1310 (PL3),

SActE_3717 (GH9),

SActE_4638,

SActE_4738 (GH16),

SActE_4755 (GH64),

SActE_5457 (GH46),

SActE_5647 (GH87), and

SActE_5978 (PL1),

and wherein the enzyme is expressed in the microbial host cell.

2. The microbial host cell of claim 1 , wherein enzyme:

SActE_0237 (GH6) comprises the amino acid sequence SEQ ID NO: 1,

SActE_0236 (GH48) comprises the amino acid sequence SEQ ID NO: 2,

SActE_3159 (CBM33) comprises the amino acid sequence SEQ ID NO: 3,

SActE_0482 (GH5) comprises the amino acid sequence SEQ ID NO: 4,

SActE_0265 (GH10) comprises the amino acid sequence SEQ ID NO: 5,

SActE_2347 (GH5) comprises the amino acid sequence SEQ ID NO: 6,

SActE_0357 (CE4) comprises the amino acid sequence SEQ ID NO: 7,

SActE_0358 (GH11) comprises the amino acid sequence SEQ ID NO: 8,

SActE_1310 (PL3) comprises the amino acid sequence SEQ ID NO: 9,

SActE_3717 (GH9) comprises the amino acid sequence SEQ ID NO: 10,

SActE_4638 comprises the amino acid sequence SEQ ID NO: 11,

SActE_4738 (GH16) comprises the amino acid sequence SEQ ID NO: 12,

SActE_4755 (GH64) comprises the amino acid sequence SEQ ID NO: 13,

SActE_5457 (GH46) comprises the amino acid sequence SEQ ID NO: 14,

SActE_5647 (GH87) comprises the amino acid sequence SEQ ID NO: 15, and

SActE_5978 (PL1) comprises the amino acid sequence SEQ ID NO: 16.

3. The microbial host cell of claim 1 , wherein enzymes SActE_0237 (GH6), SActE_0236 (GH48), SActE_3159 (CBM33), SActE_0482 (GH5), and SActE_3717 (GH9) are expressed in the microbial host cell from the at least one exogenous nucleic acid molecule.

4. The microbial host cell of claim 1 , wherein enzymes SActE_0265 (GH10), SActE_0357 (CE4), SActE_0358 (GH11), and SActE_5978 (PL1) are expressed in the microbial host cell from the at least one exogenous nucleic acid molecule.

5. The microbial host cell of claim 4 , said microbial host cell further comprising an exogenous nucleic acid molecule encoding xylose isomerase SActE_5230.

6. The microbial host cell of claim 1 , wherein enzyme SActE_2347 (GH5) is expressed in the microbial host cell from the at least one exogenous nucleic acid molecule.

7. The microbial host cell of claim 1 , wherein enzyme SActE_1310 (PL3) is expressed in the microbial host cell from the at least one exogenous nucleic acid molecule.

8. The microbial host cell of claim 1 , wherein chondroitin/alginate lyase SActE_4638 is expressed in the microbial host cell from the at least one exogenous nucleic acid molecule.

9. The microbial host cell of claim 1 , wherein enzyme SActE_5647 (GH87) is expressed in the microbial host cell from the at least one exogenous nucleic acid molecule.

10. The microbial host cell of claim 1 , wherein enzymes SActE_4738 (GH16) and SActE_4755 (GH64) are expressed in the microbial host cell from the at least one exogenous nucleic acid molecule.

11. The microbial host cell of claim 1 , wherein enzymes SActE_0237 (GH6), SActE_0236 (GH48), SActE_3159 (CBM33), SActE_0482 (GH5), SActE_0265 (GH10), SActE_2347 (GH5), SActE_0358 (GH11), SActE_1310 (PL3), and SActE_3717 (GH9) are expressed in the microbial host cell from the at least one exogenous nucleic acid molecule.

12. The microbial host cell of claim 1 , wherein said microbial host cell is selected from the group consisting of Streptomyces lividans, Trichoderma reesei, Saccharomyces cerevisiae , and Escherichia coli.

13. A Streptomyces sp. ActE strain host cell comprising at least one exogenous nucleic acid molecule encoding a Streptomyces sp ActE (SActE) enzyme, wherein said enzyme is selected from the group consisting of: