Methods and reagents that specifically detect, distinguish and quantify IFN-LAMBDA2 mRNA from IFN-LAMBDA3 mRNA in humans
The present invention provides a method of specifically detecting IFN-λ2 mRNA or IFN-λ3 mRNA. There is provided a qRT-PCR method specifically detecting, discriminating and quantifying IFN-λ2 and IFN-λ3 mRNA in a biological sample obtained from a human. There is provided qRT-PCR methods and primers and probes that specifically detect IFN-λ2 mRNA but not IFN-λ3 mRNA and vice versa in humans in order to detect, quantify and discriminate IFN-λ2 mRNA and IFN-λ3 mRNA.
1. A kit suitable for quantifying IFN-λ2 mRNA, comprising:
a. a IFN-λ2-specific forward primer, said forward primer consisting of SEQ ID NO: 3;
b. a IFN-λ2-specific reverse primer, said reverse primer consisting of SEQ ID NO: 4;
c. a IFN-λ2-specific positive control, said positive control consisting of SEQ ID NO: 1; and
d. a IFN-λ2-specific negative control, said negative control consisting of SEQ ID NO: 2; and
e. an instruction for performing a qRT-PCR to specifically quantify IFN-λ2 in a biological sample.
2. A kit suitable for quantifying IFN-λ3 mRNA, comprising:
a. a IFN-λ3-specific forward primer, said forward primer consisting of SEQ ID NO: 10;
b. a IFN-λ3-specific reverse primer, said reverse primer consisting of SEQ ID NO: 11;
c. a IFN-λ3-specific positive control, said positive control consisting of SEQ ID NO: 7; and
d. a IFN-λ3-specific negative control, said negative control consisting of SEQ ID NO: 6; and
e. an instruction for performing a qRT-PCR to specifically quantify IFN-λ3 in a biological sample.
3. A kit suitable for quantifying IFN-λ2 mRNA from IFN-λ3 mRNA, comprising:
a. a IFN-λ2-specific forward primer, said forward primer consisting of SEQ ID NO: 3;
b. a IFN-λ2-specific reverse primer, said reverse primer consisting of SEQ ID NO: 4;
c. a IFN-λ2-specific positive control, said positive control consisting of SEQ ID NO: 1;
d. a IFN-λ2-specific negative control, said negative control consisting of SEQ ID NO: 2;
e. a IFN-λ3-specific forward primer, said forward primer consisting of SEQ ID NO: 10;
f. a IFN-λ3-specific reverse primer, said reverse primer consisting of SEQ ID NO: 11;
g. a IFN-λ3-specific positive control, said positive control consisting of SEQ ID NO: 7;
h. a IFN-λ3-specific negative control, said negative control consisting of SEQ ID NO: 6; and
i. an instruction for performing a qRT-PCR to specifically quantifying IFN-λ2 mRNA from IFN-λ3 mRNA in a biological sample.