Gelatin purification
Described is an improved method of removing lipopolysaccharide from an aqueous medium comprising gelatin and lipopolysaccharides, the method comprising the steps of providing an aqueous medium comprising gelatin and lipopolysaccharides, adding to the aqueous medium a micelle-forming surfactant, contacting the medium with a solid adsorbent, separating the solid adsorbent of from the medium and recovering the aqueous medium comprising the gelatin, wherein the method is performed at conditions below the cloud point of the surfactant.
1. A method of removing lipopolysaccharide from an aqueous medium comprising gelatin and lipopolysaccharides, said method comprising the steps of:
1) providing an aqueous medium comprising at least 2 w/w % gelatin and lipopolysaccharides,
2) adding to the aqueous medium 0.01-1.5 w/w % of a micelle-forming surfactant,
3) contacting the medium of step 2) with a solid adsorbent,
4) separating the solid adsorbent of step 3) from the medium,
5) recovering the aqueous medium comprising the gelatin, wherein each of the steps 1)-5) are performed at a temperature of 68° C. or less, said temperature being below the cloud point of the micelle-forming surfactant, at least steps 2) and 3) being performed at a temperature of at least 30° C.
2. The method of 1 , wherein the surfactant is represented by the formula
whereby n=on average 9.5 or
whereby n=on average 12.5, or mixtures thereof.
3. The method of claim 1 , wherein the solid adsorbent comprises activated carbon.
4. The method of claim 1 , wherein each of the steps 1)-5) are performed at a temperature of 60° C. or less.
5. The method of claim 1 , wherein at least steps 2) and 3) are performed at a temperature of at least 45° C.
6. The method of claim 1 , wherein each of steps 1)-5) are performed at a temperature of at least 45° C.
7. The method of claim 1 , wherein the pH of the medium in step 1) is between 3.5 and 9.0.
8. The method of claim 1 , wherein the aqueous medium in step 1) comprises at least 8 w/w % dissolved gelatin.
9. The method of claim 1 , wherein the weight ratio of gelatin to added micelle-forming surfactant is 50-5:1.
10. The method of claim 1 , wherein step 2) comprises incubating the medium for 1 minute to 1 hour after adding the surfactant.
11. The method of claim 1 , wherein the method is free of a centrifugation step.
12. The method of claim 1 , wherein the aqueous medium has a salt content 100 mM or less during steps 1)-5).
13. The method of claim 1 , wherein the aqueous medium is free of acetone and/or alcohol in steps 1)-5).
14. The method of claim 1 , wherein the aqueous medium in step 1) has a lipopolysaccharide content of 1500 EU/g dry weight of gelatin or less.
15. Aqueous medium comprising at least 2 w/w % gelatin obtainable by the method according to claim 9 , said medium having a salt content of 100 mM or less, the aqueous medium being free of acetone and quaternary ammonium salts.