Flat-field imaging system and methods of use
A method of aligning a plurality of targets is provided. The method includes generating a plurality of targets. A third phase includes the plurality of targets. The method further includes combining a first phase, a second phase, and the third phase in a volume. The first phase, the second phase, and the third phase are substantially immiscible, and the third phase is in fluid communication with the first phase and the second phase, and the first phase, the second phase, and the third phase are operable to be in a configuration of the third phase between the first phase and the second phase in the volume.
1. A method for imaging droplets comprising biological component or sample, the method comprising:
forming a volume, wherein the volume comprises:
a first phase,
a second phase, and
a third phase, wherein:
the first phase, the second phase, and the third phase are substantially immiscible,
the third phase is in fluid communication with the first phase and the second phase and is within a field of view of an optical sensor, and
the third phase includes a plurality of droplets comprising biological component or sample; and
simultaneously imaging, using an optical sensor, the plurality of droplets, wherein the plurality of droplets are disposed in a substantially non-overlapping single layer within the volume during the imaging.
2. The method of claim 1 , wherein the first phase has a first density, the second phase has a second density, and the third phase as a third density, wherein first density is heavier than the third density and the third density is heavier than the second density.
3. The method of claim 2 , wherein the first phase is positioned below the third phase and the third phase is positioned below the second phase.
4. The method of claim 2 , wherein the plurality of droplets have substantially the same density as the third density.
5. The method of claim 1 , wherein the first phase comprises a fluorinated fluid (HFE), and the second phase comprises a mineral oil.
6. The method of claim 1 , wherein the plurality of droplets comprises droplets of a plurality of sizes.
7. A system for imaging droplets comprising biological component or sample, the system comprising:
a volume, wherein the volume comprises:
a first phase,
a second phase, and
a third phase, wherein:
the first phase, the second phase, and the third phase are substantially immiscible,
the third phase is configured to be positioned between the first phase and the second phase, and
the third phase includes a plurality of droplets comprising biological component or sample, the plurality of droplets being disposed in a substantially non-overlapping single layer within the volume; and
an optical sensor having a field of view including the third phase for simultaneously imaging the plurality of droplets in the volume.
8. The system of claim 7 , wherein the volume is contained in a vessel.
9. The system of claim 7 , wherein the optical sensor is positioned above the volume.
10. The system of claim 7 , wherein the optical sensor is positioned below the volume.
11. The system of claim 7 , wherein the first phase has a first density, the second phase has a second density, and the third phase as a third density, wherein first density is heavier than the third density and the third density is heavier than the second density.
12. The system of claim 11 , wherein the first phase is positioned below the third phase and the third phase is positioned below the second phase.
13. The system of claim 11 , wherein the plurality of droplets have substantially the same density as the third density.
14. The system of claim 7 , wherein the second phase comprises a fluorinated fluid (HFE), and the first phase comprises a mineral oil.
15. The system of claim 7 , wherein the plurality of droplets comprises droplets of a plurality of sizes.
16. The system of claim 7 , wherein the third phase further includes a medium that is at least substantially immiscible with the plurality of droplets.
17. The system of claim 16 , wherein the medium that is substantially immiscible with the plurality of droplets comprises at least one selected from the group consisting of: a mineral oil, a silicone oil, a paraffin oil, a fluorinated fluid, a perfluorinated polyether.
18. The system of claim 7 , wherein at least some of the plurality of droplets contain a porous beads.
19. The system of claim 7 , wherein the plurality of droplets comprises magnetic beads.