Fluorescent detector systems for the detection of chemical perturbations in sterile storage devices
System and method for detecting and measuring chemical perturbations in a sample. The system and method are useful for non-invasive pH monitoring of blood or blood products sealed in storage bags.
1. A method for monitoring a parameter of one or more samples positioned in an incubator, comprising:
(a) irradiating a fluorescent species having a first emission intensity at a first emission wavelength and a second emission intensity at a second emission wavelength, wherein the first and second emission wavelengths are not the same, each emission intensity dependent on a parameter, with excitation light emanating from a probe physically isolated from the fluorescent species to provide first and second emission intensities, wherein the fluorescent species is in a sample contained in a vessel in an incubator;
(b) measuring the first and second emission intensities to determine a first parameter reading of the sample; and
(c) repeating step (a) after a pre-determined time and measuring the first and second emission intensities to determine a second parameter reading of the sample.
2. The method of claim 1 , wherein step (c) is repeated to provide multiple parameter readings to monitor the parameter of the sample over time.
3. The method of claim 1 , wherein the parameter is pH.
4. The method of claim 1 , wherein the parameter is CO 2 .
5. The method of claim 1 , wherein the parameter is oxygen.
6. The method of claim 1 , wherein the parameter is glucose.
7. The method of claim 1 further comprising measuring the first and second emission intensities of each sample positioned in the incubator to determine a first parameter reading for each of the samples.
8. The method of claim 7 , wherein step (c) is repeated to provide multiple parameter readings to monitor the parameter of each of the samples over time.
9. A method for monitoring the pH of one or more samples positioned in an incubator, comprising:
(a) irradiating a fluorescent species in a sample contained in a vessel with excitation light emanating from a probe physically isolated from the fluorescent species, wherein the excitation light has a wavelength sufficient to effect fluorescent emission from the fluorescent species, wherein the fluorescent species exhibits a first emission intensity at a first emission wavelength and a second emission intensity at a second emission wavelength, the ratio of the first and second emission intensities being dependent on pH, wherein the first and second emission wavelengths are not the same;
(b) measuring the first and second emission intensities to determine the first pH of the sample in the incubator; and
(c) repeating step (a) after a pre-determined time and measuring the first and second emission intensities to determine the second pH of the sample in the incubator.
10. The method of claim 9 , wherein step (c) is repeated to provide multiple pH readings to monitor the pH of the sample over time.
11. The method of claim 9 further comprising measuring the first and second emission intensities of each sample positioned in the incubator to determine a first parameter reading for each of the samples.
12. The method of claim 9 , wherein step (c) is repeated to provide multiple parameter readings to monitor the parameter of each of the samples over time.