Methods for amplification of nucleic acids on solid support
View Patent ↗The present invention provides methods for amplifying a nucleic acid from a sample containing a mixture of nucleic acids utilizing a solid support. Methods are provided utilizing user-defined primer oligonucleotides for directional amplification that assists in further manipulation of the target nucleic acid, such as sequencing. Methods are also provided utilizing blocker and displacer oligonucleotides for generating amplified target nucleic acids of defined length. One of these methods provides a first oligonucleotide and a second oligonucleotide affixed to a solid support or separate solid supports. The first oligonucleotide is blocked to prevent extension from the 3′-terminus and has a sequence complementary to a first portion of a target nucleic acid. The second oligonucleotide has a sequence that is identical to a second portion of the target nucleic acid. In this method, a sample is applied to the solid support and the target nucleic acid within the sample binds said first oligonucleotide. The solid support is then washed to remove unbound nucleic acids. A primer sequence containing a target binding region and a polymerase promoter sequence is then annealed to the bound target nucleic acid and extended producing a first duplex nucleic acid. The target sequence is then removed leaving a first nucleic acid that can now bind the second oligonucleotide. The second oligonucleotide is extended to produce a second duplex nucleic acid that contains a second nucleic acid. The second nucleic acid is then amplified by adding a polymerase.
1. A method of immobilizing and amplifying a target nucleic acid from a sample containing a mixture of nucleic acids, said method comprising the steps of:
applying said sample to a solid support, said solid support having a first oligonucleotide and a second oligonucleotide affixed thereto, wherein said first oligonucleotide is blocked to prevent extension from the 3′-terminus and has a sequence complementary to a first portion of said target nucleic acid and said second oligonucleotide has a sequence that is identical to a second portion of said target nucleic acid, wherein said target nucleic acid binds said first oligonucleotide and optionally washing said solid support to remove unbound nucleic acids;
hybridizing a primer to said target nucleic acid wherein said primer contains a target binding region and a polymerase promoter sequence and extending said primer by polymerase to produce a first duplex nucleic acid and optionally removing said target nucleic acid from said duplex nucleic acid to produce a first nucleic acid;
annealing said first nucleic acid to said second oligonucleotide and extending said second oligonucleotide by polymerase to produce a second duplex nucleic acid containing said first nucleic acid and a second nucleic acid; and
generating multiple copies of a third nucleic acid by polymerase, thereby amplifying said target nucleic acid.
2. The method according to claim 1 , further comprising the steps of:
annealing said amplified third nucleic acid with said second oligonucleotide and extending said second oligonucleotide to produce a third duplex nucleic acid;
optionally removing said amplified third nucleic acid to produce a solid support bound fourth nucleic acid;
annealing said primer sequence to said fourth nucleic acid and extending said primer and said fourth nucleic acid by polymerase to produce a fourth duplex nucleic acid containing a fifth nucleic acid and additional second nucleic acid; and
generating multiple copies of said third nucleic acid by polymerase, said polymerase being specific to a promoter on said fourth duplex nucleic acid thereby amplifying said target nucleic acid.
3. The method according to claim 1 , wherein said primer further comprises a tag sequence comprising one or more non-natural nucleotides.
4. The method according to claim 1 , wherein said second oligonucleotide is four oligonucleotides each with a different nucleotide at the 3′-terminus specific for four different SNPs of said first nucleic acid.