Probiotic and prebiotic compositions
The invention relates to products and compositions that may be beneficial in animal husbandry. Said products and compositions comprise microorganisms, such as bacteria, and probiotic bacteria in particular. Thus, provided herein are microbial strains, as well as selection criteria which will enable the skilled reader to find further strains useful in the present invention. The strains, as well as compositions comprising the same, may be administered to animals, farmed animals such as swine in particular. The administration may occur in the first days of life. By administration of the products or compositions of the inventions animal growth can. be promoted and animal weight can be increased. Infections may also be prevented or treated by said compounds or compositions.
1. A method for treating or preventing diarrhea caused by a viral infection in a subject in need thereof, comprising administering to said subject a composition comprising a Lactobacillus reuteri bacterial strain deposited at the Spanish Type Cultures Collection on Sep. 10, 2014 under deposit number CECT 8700 (AqSynRMH69).
2. The method of claim 1 , wherein the composition further comprises at least one additional bacterial strain, wherein the bacterial strain has at least one of the following antimicrobial activities, as evidenced by inhibition zones determined by the spot on lawn assay: (i) a 10 mm or greater inhibition zone for Salmonella , (ii) a 9 mm or greater inhibition zone for Listeria monocytogenes , (iii) a 9 mm or greater inhibition zone for Staphyloccocus aureus , or (iv) a 10 mm or greater inhibition zone for Escherichia coli.
3. The method of claim 2 , wherein the at least one additional bacterial strain belongs to a genus selected from the group consisting of Lactobacillus, Leuconostoc, Pediococcus, Lactococcus, Streptococcus Aerococcus, Carnobacterium, Enterococcus, Oenococcus, Sporolactobacillus, Tetragenococcus, Vagococcus and Weisella.
4. The method of claim 1 , wherein the composition further comprises a Lactobacillus plantarum bacterial strain deposited at Spanish Type Cultures Collection on May 16, 2013 under deposit number CECT 8350 (AqSynJ59).
5. The method of claim 4 , wherein the composition comprises only two bacterial strains, and wherein the two bacterial strains are the bacterial strain AqSynRMH69 and the bacterial strain AqSynJ59.
6. The method of claim 5 , wherein the bacterial strain AqSynRMH69 and the bacterial strain AqSynJ59 are present in the composition at a ratio of AqSynRMH69:AqSynJ59 of from 2:1 to 1:2 as measured by colony forming units (CFU).
7. The method of claim 2 , wherein at least one bacterial strain at least one strain, and comprised in the composition is free from antibiotic resistance.
8. The method of claim 2 , wherein at least one bacterial strain comprised in the composition retains essentially the same viability after a 3 hour incubation at pH 3.5, wherein retaining essentially the same viability after the incubation comprises generating at least 50% of the number of CFU after the incubation as compared to the number of CFU generated before the incubation.
9. The method of claim 2 , wherein at least one bacterial strain comprised in the composition retains essentially the same viability after a 4 hour of incubation in the presence of a 0.45% bile extract, wherein retaining essentially the same viability after the incubation comprises generating at least 50% of the number of CFU after the incubation as compared to the number of CFU generated before the incubation.
10. The method of claim 2 , wherein at least one bacterial strain comprised in the composition has all of the following antimicrobial activities, as evidenced by inhibition zones determined by the spot on lawn assay: (i) a 10 mm or greater inhibition zone for Salmonella , (ii) a 9 mm or greater inhibition zone for Listeria monocytogenes , (iii) a 9 mm or greater inhibition zone for Staphyloccocus aureus , (iv) a 10 mm or greater inhibition zone for Escherichia coli.
11. A method for treating or preventing diarrhea caused by a viral infection in a subject in need thereof, comprising administering to said subject a composition comprising a mixture of bacterial strains, wherein the bacterial strains belong to the bacterial species Lactobacillus plantarum and Lactobacillus reuteri , wherein the bacterial strains have at least one of the following antimicrobial activities, as evidenced by inhibition zones determined by the spot on lawn assay: (i) a 10 mm or greater inhibition zone for Salmonella , (ii) a 9 mm or greater inhibition zone for Listeria monocytogenes , (iii) a 9 mm or greater inhibition zone for Staphyloccocus aureus , (iv) a 10 mm or greater inhibition zone for Escherichia coli ; and
wherein the composition is administered as at least one dose within the first 48 hours after birth of the subject.
12. The method of claim 11 , wherein the composition further comprises at least one bacterial strain belonging to the genera selected from the group consisting of Lactobacillus, Leuconostoc, Pediococcus, Lactococcus, Streptococcus, Aerococcus, Carnobacterium, Enterococcus, Oenococcus, Sporolactobacillus, Tetragenococcus, Vagococcus and Weisella,
wherein the composition does not comprise a Lactobacillus fermentum bacterial strain deposited at the Spanish Type Cultures Collection on May 16, 2013 under deposit number CECT 8347 (AqSynJ12) or a Lactobacillus mucosae bacterial strain deposited at the Spanish Type Cultures Collection on May 16, 2013 under deposit number CECT 8349 (AqSynJ55).