Nucleic acids encoding fusion proteins comprising PDGF and VEGF binding portions and methods of using thereof
The present provides fusion proteins comprising PDGF and VEGF binding portions, and recombinant viral particles encoding the fusion proteins. Compositions comprising the fusion proteins and viral particles as well as methods of using the same are also provided.
1. A nucleic acid encoding a fusion protein wherein the fusion protein comprises (a) an extracellular portion of a PDGF receptor beta (PDGFRβ) comprising Ig-like domains D1-D3, (b) an extracellular portion of a VEGF receptor 1 (FLT-1) comprising an Ig-like domain D2, and (c) a multimerization domain, wherein the fusion proteins binds to a PDGF and a VEGF, and the fusion protein is arranged from N-terminus to C-terminus in the following order: (a), (b) and (c), and wherein the fusion protein does not comprise an Ig-like domain D3 of a VEGFR2.
2. A host cell comprising the nucleic acid of claim 1 .
3. A method of producing a fusion protein, comprising culturing a host cell comprising the nucleic acid of claim 1 under a condition that produces the fusion protein, and recovering the fusion protein produced by the host cell.
4. The method of claim 3 , wherein the host cell is a mammalian cell.
5. A vector comprising the nucleic acid of claim 1 .
6. The vector of claim 5 , which is a viral vector.
7. The vector of claim 6 , wherein the viral vector is a recombinant adeno-associated virus vector (rAAV).
8. The vector of claim 7 , wherein the rAAV vector comprises an ITR of AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh8, or AAVrh10.