IP Library › Granted Patent US 10,184,143
Granted Patent B2
US 10,184,143 · App. 15/285,392 · Granted Jan 22, 2019

Methods for increasing mannose content of recombinant proteins

Inventors: Chung-Jr Huang (Newbury Park, CA); Xiaoming Yang (Oak Park, CA)
Assignee: Amgen Inc.
C12P21/005C07K16/00C12N5/0018C07K2317/41C12N2500/34
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Quick Facts
Patent No.
US 10,184,143
App. No.
15/285,392
Granted
Jan 22, 2019
Kind
B2
Abstract

The present invention relates to methods of upregulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture by manipulating the mannose to total hexose ratio in the cell culture media formulation.

Claims (2)

1. A serum-free perfusion media containing mannose, wherein the mannose to total hexose ratio in the serum-free perfusion media is greater than 0 but less than 1.0.

2. The serum-free perfusion media according to claim 1 , wherein the serum-free perfusion media contains at least 3 g/L mannose.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 9, 2017
From: HUANG, CHUNG, JR.; YANG, XIAOMING
To: AMGEN INC.
Reel/Frame 040907/0640 →
Continuity (3)
Division 14289943 · May 29, 2014
Provisional Application 61828969 · May 30, 2013
Related Publication 20170088874A1 · Mar 30, 2017