Aptamers against EGFR and therapeutic uses thereof
The invention relates to novel aptamers, in particular aptamers which are capable of binding to EGFR. The invention also relates to cancer cell binding complexes comprising said aptamers and the use of said cancer cell binding complexes in the treatment of cancer.
1. A ribonucleic acid molecule comprising a nucleotide sequence:
5′-mGmG mG mAfUfU fUAA fUfCmG fCfCmG fUmAmG AmAmA AmGfC mAfUmG fUfCmA AAmG fCfCmG mGmAA fCfCfC-3′ (ALT102139.01) (SEQ ID NO: 79), wherein “m” represents a 2′-O-methyl substituted nucleotide and “f” represents a 2′-fluoro substituted nucleotide.
2. The ribonucleic acid molecule as defined in claim 1 , which additionally comprises a 3′ capping modification.
3. The ribonucleic acid molecule as defined in claim 2 , which additionally comprises an addition of an inverted thymidine nucleotide (i-dT) at the 3′ end of said ribonucleic acid molecule.
4. The ribonucleic acid molecule as defined in claim 1 , wherein said one or more additional nucleotides comprise an additional AAA or AAAG nucleotide motif.
5. The ribonucleic acid molecule as defined in claim 4 , wherein all of said additional nucleotides contain a 2′-OMe substitution.
6. The ribonucleic acid molecule as defined in claim 5 , wherein said molecule has the sequence of:
(ALT102145.01)
(SEQ ID NO: 83)
5′-mAmAmA GGA fCGG AfUfU fUAA fUfCG fCfCG fUAG AAA
AGfC AfUG fUfCA AAG fCfCG GAA fCfCG fUfCfC-3′.
7. The ribonucleic acid molecule as defined in claim 4 , wherein all of said additional nucleotides contain a 2′-OMe substitution and said molecule has the sequence of:
(ALT102146.01)
(SEQ ID NO: 84)
5′-mAmAmAmG mGmG mAfUfU fUAA fUfCmG fCfCmG
fUmAmG AmAmA AmGfC mAfUmG fUfCmA AAmG fCfCmG
mGmAA fCfCfC-3′.
8. The ribonucleic acid molecule as defined in claim 4 , wherein said one or more additional nucleotides comprise an additional AAA or AAAG nucleotide motif at the 5′ end.
9. A cancer cell binding complex which comprises a ribonucleic acid molecule as defined in claim 1 conjugated to an immunogenic molecule.
10. The cancer cell binding complex as defined in claim 9 , wherein the immunogenic molecule is selected from: the alpha-Gal epitope (i.e. galactosyl-alpha-1,3-galactosyl-beta-1,4-N-acetylglucosamine), dinitrophenyl (DNP) and L-rhamnose.
11. The cancer cell binding complex as defined in claim 9 , wherein the immunogenic molecule is the alpha-Gal epitope (i.e. galactosyl-alpha-1,3-galactosyl-beta-1,4-N-acetylglucosamine).
12. A pharmaceutical composition comprising at least one cancer cell binding complex as defined in claim 9 , together with one or more pharmaceutically acceptable excipients.
13. The pharmaceutical composition as defined in claim 12 further comprising other therapeutic or prophylactic agents.
14. A method for the treatment of cancer in a subject comprising:
administering a pharmaceutical composition comprising at least one cancer binding complex as defined in claim 9 to the subject.