Vaccination strategy
In a first aspect, the present invention relates to the use of a TLR 9 agonist and/or a TLR 4 agonist in a prophylactic or therapeutic vaccine. According to the present vaccination strategy, the TLR 9 agonist and/or TLR 4 agonist is adapted or designed for use as a multiplying jump agent to enhance numbers and functionality of CD8 T cells in a prime-jump vaccination strategy for jump T cell expansion. In particular, the TLR 9 agonist and/or TLR 4 agonist is used as a component to be administered after priming of the individual to be vaccinated. The vaccination strategy is particularly useful against acute and chronic infections with intracellular pathogens or for anti-tumor vaccination. In another aspect, the present invention relates to a kit of part containing a prime agent and a multiplying jump agent according to the present invention.
1. A method for expansion of effector CD8 T cells comprising the steps of:
administering a priming agent,
separately administering a TLR9 agonist at least 6 days after the administration of the priming agent; and
wherein the TLR9 agonist is administered as a multiplying jump agent enhancing the number and functionality of CD8 T cells.
2. The method for expansion of effector CD8 T cells according to claim 1 wherein the TLR9 agonist is administered with a time range of 10 to 20 days after administration of the priming agent.
3. The method for expansion of effector CD8 T cells according claim 1 for vaccination against infection with an intracellular pathogen or cancer.
4. The method for expansion of effector CD8 T cells against infection with an intracellular pathogen according to claim 3 wherein the intracellular pathogen is a viral pathogen, an intracellular bacterial pathogen or an intracellular parasite.
5. The method for expansion of effector CD8 T cells according to claim 1 wherein the vaccination is against a chronic or acute infection.
6. The method for expansion of effector CD8 T cells against a chronic or acute infection according to claim 5 when the chronic or acute infection is a chronic or acute viral infection.
7. The method for expansion of effector CD8 T cells according claim 1 wherein the TLR9 agonist is selected from CpG oligonucleotide, in particular, a CpG oligodeoxynucleotide.
8. The method for expansion of effector CD8 T cells according to claim 1 wherein administration of the TLR9 agonist is intravenously or to a vascularized area.
9. The method for expansion of effector CD8 T cells according to claim 1 wherein the TLR9 agonist is administered in a composition in which the TLR9 agonist is the only active ingredients.
10. The method for expansion of effector CD8 T cells according to claim 9 wherein the priming agent is an antigenic compound stemming from an intracellular pathogen to be vaccinated against allowing secondary CD8 T cell expansion, in particular, expansion of effector CD8 T cells.