Small molecules for endothelial cell activation

1. A pharmaceutically acceptable pro-inflammatory composition comprising a pharmaceutically acceptable carrier and an effective amount of one or more of octahydro-1,6-naphthyridin-4-one or a pro-inflammatory analog for augmenting innate immune responses in a mammal, wherein the one or more octahydro-1,6-naphthyridin-4-one or pro-inflammatory analog thereof is octahydro-1,6-naphthyridin-4-one, a compound represented by the formula 104A5, 104A7, 104A8, 104B9, 104B10, 104B11, 104C2, 104C3, 104C4, 104C5, 104C6, 104E2, 104E4, 104E6, 104F8, 104F9, 104F11, 104G2, 104H3, 104H4, 104H5, 104H6, 104H7, 104H8, 104H9, 104H10, 104H11, 105A3, 105A6, 105A7, 105A8, 105A9, 105A10, 105A11, 105B2, 105B3, G2, G3, G4, G6, G7, G8, G10, G11, H2, H3, H4, H5, H6, H7, H8, H9, H10, 2A2, 2A3, 2A4, 2A5, 2A6, 2A7, 2A8, 2A9, 2A10, 2A11, 2B3, 2B4, 2B5, 2B6, 2B7,

or pharmaceutically acceptable salt thereof.

2. A method of preparing the pharmaceutically acceptable pro-inflammatory composition of claim 1 , comprising preparing the octahydro-1,6-naphthyridin-4-one compound or analog, wherein preparing the octahydro-1,6-naphthyridin-4-one compound or analog comprises:

forming an enol ether intermediate via phosphine-catalyzed [4+2] annulation of an allenoate with a first imine building block followed by treatment with Tebbe reagent and anhydrous pyridine to form an enol ether intermediate,

subjecting the enol ether intermediate to endo-selective Diels-Alder reaction with a second imine building block to yield an octahydro-1,6-naphthyridine intermediate, and

forming the octahydro-1,6-naphthyridine-4-one compound or analog.

3. The method of claim 2 , wherein the allenoate is formed by coupling a Wang resin having reactive hydroxyl groups with 2-methyl-2,3-butadienoic acid, and wherein the method is solid phase synthesis.

4. The method of claim 3 , wherein the solid phase synthesis is carried out via combinatorial library construction.

5. The method of claim 4 , wherein the solid support comprises Synphase lanterns wherein the first imine building block is encoded by tagging individual lanterns.

6. A pharmaceutically acceptable pro-inflammatory composition comprising a pharmaceutically acceptable carrier and an effective amount of one or more octahydro-1,6-naphthyridin-4-one analog represented by the formula 105A3-B2:

wherein:

Ar=phenyl, 4-MeC 6 H 4 , 4-EtC 6 H 4 , 4-ClC 6 H 4 , 3-ClC 6 H 4 , 2-thiophenyl, or 1-naphthyl;

P=tosyl, or benzenesulfonyl;

Ar′=phenyl, 4-EtC 6 H 4 , 4-ClC 6 H 4 , or 2-thiophenyl,

or a pharmaceutically acceptable salt thereof for augmenting innate immune responses in a mammal.

7. The pharmaceutically acceptable pro-inflammatory composition of claim 6 , wherein:

Ar=4-EtC6H4;

P=tosyl; and

Ar′=4-EtC6H4,

or a pharmaceutically acceptable salt thereof.