Method for preventing spontaneous oxidation of antioxidant using aptamer, aptamer-based control of the release rate of active ingredient in the hydrogel, material and use thereof
The present disclosure relates to a method for preventing spontaneous oxidation of an antioxidant, material thereof and uses thereof. More particularly, the present disclosure relates to a method for preventing oxidation of an antioxidant using aptamer specifically binding to its target antioxidant and aptamer-based control of the release rate of active ingredient in the hydrogel. Aptamer having such activity can have versatile applications such as cosmeceuticals and health beverages. As an example, we provide in the present disclosure the establishing method of aptamer targeting for Vitamin C and verification of its prevention of spontaneous oxidation of Vitamin C. We also provide the detailed method for trapping such aptamer-active ingredient complex in the hydrogel. It is expected that an aptamer-trapped hydrogel of the present disclosure has functions of controlling a release rate of the active ingredient through the aptamer-based sensing of specific substance released from the skin according to the skin conditions.
1. A method of preventing oxidation of vitamin C by treating the vitamin C with an aptamer, wherein the aptamer includes a base sequence as set forth in SEQ ID NO:1.
2. A method for preparing an aptamer-trapped hydrogel, the method comprising the steps of:
g) binding an amine group to the aptamer;
h) silanizing a hydroxyl group of a hydrogel monomer with 3-glycidoxypropyltrimethoxysilane (3-GPTMS) having an epoxy group, and then binding an amine group binding to the aptamer to the epoxy group; and
i) polymerizing the hydrogel monomer, wherein the aptamer includes a base sequence as set forth in SEQ ID NO:1.