IP Library › Granted Patent US 10,202,598
Granted Patent B2
US 10,202,598 · App. 15/349,886 · Granted Feb 12, 2019

Methods and systems for generation, use, and delivery of activated stem cells

Inventors: Todd Frank Ovokaitys (Carlsbad, CA); John Scott Strachan (Edinburgh, GB)
Assignee: Todd Frank Ovokaitys
C12N13/00A61K35/28A61K35/51A61K41/00A61N5/062A61N5/0622A61N2005/067A61N2005/0626A61N2005/0659A61N2005/0662
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Quick Facts
Patent No.
US 10,202,598
App. No.
15/349,886
Granted
Feb 12, 2019
Kind
B2
Abstract

Harvested stem cells are activated by treating them with an amplitude modulated laser beam having a wavelength lying in the range of 405 to 980 nanometers. The frequency of the laser beam is modulated within a range of 8 to 12 MHz. Using the activated stem cells, tissue can be repaired and regenerated by preparing the unactivated stem cells, treating the unactivated stem cells with an amplitude modulated laser beam having a pre-determined frequency for obtaining activated stem cells, administering the activated stem cells into a body containing the tissue, and using a homing beam to guide the activated stem cells within the body to the location of the tissue.

Claims (35)

1. A method of repairing damaged biological tissue comprising:

obtaining unactivated stem cells;

forming activated stem cells from the unactivated stem cells by treating the stem cells with an amplitude modulated laser beam having a pre-defined wavelength and a pre-defined amplitude;

administering the activated stem cells into a body containing the biological tissue;

transcutaneously applying a homing coherent laser beam along a first axis such that the homing coherent laser beam interacts with said damaged biological tissue; and

transcutaneously applying the homing coherent laser beam along a second axis such that the homing coherent laser beam interacts with the damaged biological tissue, wherein the first axis and the second axis intersect at the damaged biological tissue and point at different directions relative to each other.

2. The method of claim 1 wherein the homing coherent laser beam is generated using a 20% to 90% phase cancellation.

3. The method of claim 1 wherein the unactivated stem cells are harvested from an autologous source including at least one of peripheral blood, bone marrow, or fat.

4. The method of claim 1 , wherein the unactivated stem cells are harvested from an exogenous source including at least one of a cord blood and a placenta of the patient.

5. The method of claim 1 , wherein the unactivated stem cells are sourced from a genetically matched stem cell donor.

6. The method of claim 1 wherein the pre-defined wavelength is in a range of 405 to 980 nanometers.

7. The method of claim 1 wherein the amplitude modulated laser beam has a wavelength of 674 nanometers.

8. The method of claim 1 , wherein the amplitude modulated laser beam comprises a string of short duration pulses of sub-femto second duration.

9. The method as claimed in claim 1 wherein, prior to treating the unactivated stem cells, the amplitude modulated laser beam is expanded in a range varying between two times to seven times by passing the amplitude modulated laser beam through a beam expander.

10. The method as claimed in claim 1 wherein, prior to treating the unactivated stem cells, the amplitude modulated laser beam is passed through a Strachan-Ovokaitys Node Generator.

11. The method as claimed in claim 1 wherein a phase cancellation of the amplitude modulated laser beam is adjusted to achieve a predetermined power output before treating the unactivated stem cells.

12. The method as claimed in claim 11 wherein the unactivated stem cells are positioned in a container and wherein the container is rotated at a speed of one rotation in every 3 to 5 seconds and is moved up and down for a duration of 15 seconds in each direction.

13. The method as claimed in claim 1 wherein treating the unactivated stem cells comprises applying the amplitude modulated laser beam to a container containing the unactivated stem cells such that the container is rotated and simultaneously moved up and down in a vertical direction during the activation process.

14. The method as claimed in claim 1 wherein treating the stem cell is with the amplitude modulated laser beam is done such that, relative to the unactivated stem cells, the activated stem cells comprise at least one of an increased expression of an alpha or beta integrin, an increase in CD34, or an enhanced migratory action in a direction of the homing coherent laser beam.

15. The method of claim 1 wherein the biological tissue is at least one of myocardial tissue, lung tissue, kidney tissue, blood vessels, immune system cells, bone tissue, teeth, liver tissue, endocrine tissues, pituitary tissue, thymus tissue, intervertebral discs, brain tissue, spinal tissue, pancreatic tissue and nerve tissue.

16. A method of treating damaged cardiac tissue of a patient comprising:

obtaining unactivated stem cells;

forming activated stem cells from the unactivated stem cells by treating the stem cells with an amplitude modulated laser beam having a pre-defined wavelength and a pre-defined amplitude;

intravenously administering the activated stem cells into the patient;

transcutaneously applying a homing laser beam along a first axis such that the homing laser beam interacts with said cardiac tissue; and

transcutaneously applying the homing laser beam along a second axis such that the homing laser beam interacts with the cardiac tissue, wherein the first axis and the second axis intersect at the cardiac tissue and point at different directions relative to each other.

17. The method of claim 16 wherein the homing laser beam is generated using a 20% to 90% phase cancellation.

18. A method of treating a neurological condition in a patient comprising:

obtaining unactivated stem cells;

forming activated stem cells from the unactivated stem cells by treating the stem cells with an amplitude modulated laser beam having a pre-defined wavelength and a pre-defined amplitude;

intravenously administering the activated stem cells into the patient;

transcutaneously applying a homing coherent laser beam along a first axis such that the homing coherent laser beam interacts with brain tissue and/or spinal cord tissue of the patient; and

transcutaneously applying the homing coherent laser beam along a second axis such that the homing coherent laser beam interacts with the brain tissue and/or spinal cord tissue, wherein the first axis and the second axis intersect at the brain tissue and/or spinal cord tissue and point at different directions relative to each other.

19. The method of claim 18 wherein the homing coherent laser beam is swept over said spinal cord tissue and swept over said brain tissue.

20. The method of claim 19 wherein the homing coherent laser beam is generated using a 20% to 90% phase cancellation.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 16, 2018
From: STRACHAN, JOHN SCOTT
To: OVOKAITYS, TODD FRANK
Reel/Frame 044631/0151 →
Continuity (5)
Continuation In Part 14726457 · May 30, 2015
Provisional Application 62321781 · Apr 13, 2016
Provisional Application 62254220 · Nov 12, 2015
Provisional Application 62006034 · May 30, 2014
Related Publication 20170233717A1 · Aug 17, 2017
Cited By (1)
US 12,246,037