Recombinant cell, and method for producing 1,4-butanediol
An object of the present invention is to provide a series of techniques for producing 1,4-butanediol from methanol or the like. Provided is a recombinant cell prepared by introducing a gene encoding at least one enzyme selected from the group consisting of succinate semialdehyde dehydrogenase, succinyl-CoA synthase, CoA-dependent succinate semialdehyde dehydrogenase, 4-hydroxybutyrate dehydrogenase, 4-hydroxybutyryl-CoA transferase, 4-hydroxybutyryl-CoA reductase, 4-hydroxybutyraldehyde dehydrogenase, and alcohol dehydrogenase, into a host cell which is a methylotroph, wherein the gene is expressed in the host cell, and the recombinant cell is capable of producing 1,4-butanediol from at least one C1 compound selected from the group consisting of methane, methanol, methylamine, formic acid, formaldehyde, and formamide.
1. A recombinant cell prepared by introducing genes encoding a group of enzymes comprising CoA-dependent succinate semialdehyde dehydrogenase, 4-hydroxybutyrate dehydrogenase, 4-hydroxybutyryl-CoA transferase, 4-hydroxybutyryl-CoA reductase, and alcohol dehydrogenase, into a host cell which is a methylotroph, wherein the recombinant cell is non-naturally occurring, wherein the methylotroph belongs to genus Methylobacterium or genus Methylophilus , and wherein the genes are expressed in said recombinant cell, which is capable of producing 1,4-butanediol from methanol and is tolerant to at least 400 mM 1,4-butanediol and tolerant to at least 2% (v/v) methanol.
2. The recombinant cell according to claim 1 , having at least one C1carbon assimilating pathway selected from the group consisting of a serine pathway, a ribulose monophosphate pathway, and a xylulose monophosphate pathway as a fixing pathway of formaldehyde.
3. The recombinant cell according to claim 1 , wherein a gene encoding 3-hexulose-6-phosphate synthase and a gene encoding 6-phospho-3-hexuloisomerase are further introduced, and the genes are expressed in the host cell.
4. A method for producing 1,4-butanediol, comprising culturing the recombinant cell according to claim 1 by using methanol as a carbon source, to cause the recombinant cell to produce 1,4-butanediol.
5. A method for producing 1,4-butanediol, comprising bringing methanol into contact with the recombinant cell according to claim 1 , to cause the recombinant cell to produce 1,4-butanediol from the methanol.