Fc fusion proteins comprising novel linkers or arrangements
The application provides Fc fusion proteins having novel arrangements. In one embodiment, the application provides Fc fusion proteins comprising a 10 F3 domain. In another embodiment, the application provides Fc fusion proteins comprising linkers derived from the naturally occurring C-terminal tail regions of membrane bound or secretory immunoglobulins.
1. An isolated nucleic acid sequence encoding a polypeptide comprising an immunoglobulin Fc domain and a heterologous polypeptide, wherein the heterologous polypeptide is fused to the N-terminus or the C-terminus of the Fc domain by a polypeptide linker, wherein the polypeptide linker comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 51-54, 63-65, and 84.
2. The nucleic acid of claim 1 , wherein the polypeptide linker comprises SEQ ID NO: 84.
3. The nucleic acid of claim 1 , wherein the heterologous polypeptide is fused to the C-terminus of the Fc domain.
4. The nucleic acid of claim 1 , wherein the heterologous polypeptide is fused to the N-terminus of the Fc domain.
5. The nucleic acid of claim 1 , wherein the heterologous polypeptide comprises a tenth fibronectin type III ( 10 Fn3) domain.
6. The nucleic acid of claim 1 , wherein the immunoglobulin Fc domain comprises a hinge or a portion thereof.
7. A vector comprising the nucleic acid of claim 1 .
8. A host cell comprising the vector of claim 7 .
9. The isolated nucleic acid sequence of claim 1 , wherein the polypeptide has one of the following arrangements from N-terminus to C-terminus: 10 Fn3 domain-hinge-Fc domain or hinge-Fc domain-linker- 10 Fn3 domain.