Incoherent fluorescence digital holographic microscopy using transmission liquid crystal lens
A new optical arrangement that creates high efficiency, high quality Fresnel Incoherent Correlation Holography (FINCH) holograms using transmission liquid crystal GRIN (TLCGRIN) diffractive lenses has been invented. This is in contrast to the universal practice in the field of using a reflective spatial light modulator (SLM) to separate sample and reference beams. Polarization sensitive TLCGRIN lenses enable a straight optical path, have 95% transmission efficiency, are analog devices without pixels and are free of many limitations of reflective SLM devices. An additional advantage is that they create an incoherent holographic system that is achromatic over a wide bandwidth. Two spherical beams created by the combination of a glass and a polarization sensitive TLCGRIN lenses interfere and a hologram is recorded by a digital camera. FINCH configurations which increase signal to noise ratios and imaging speed are also described.
1. An apparatus configured to produce a Fresnel hologram of an object emitting electromagnetic radiation, said apparatus comprising polarization sensitive liquid crystal refractive or diffractive elements, wherein a path of the electromagnetic radiation within the apparatus is free of reflective optics.
2. The apparatus of claim 1 wherein the liquid crystal refractive or diffractive elements are liquid crystal GRIN lenses.
3. The apparatus of claim 1 wherein the liquid crystal refractive or diffractive elements are liquid crystal Fresnel lenses.
4. The apparatus of claim 1 wherein a hologram of an object is formed when in combination with a non-polarized optical element.
5. The apparatus of claim 1 wherein the electromagnetic radiation is visible light.
6. The apparatus of claim 1 wherein the electromagnetic radiation is fluorescent light.
7. The apparatus of claim 1 wherein the electromagnetic radiation is X-ray.
8. The apparatus of claim 1 in which the electromagnetic radiation is split into separate p and s polarization beams and each beam is captured by a separate camera.
9. The apparatus of claim 8 in which one beam produces a widefield image and the other beam produces a hologram.
10. The apparatus of claim of 8 in which each beam creates a hologram.
11. The apparatus of claim 10 in which the holograms from each camera are combined to improve the signal to noise ratio.
12. An apparatus configured to produce a hologram of an object emitting electromagnetic radiation, said apparatus comprising polarization sensitive liquid crystal refractive or diffractive elements and input and output polarizing beam splitters, wherein a path of the electromagnetic radiation within the apparatus is free of reflective optics.
13. The apparatus of claim 12 wherein a separate hologram is produced by the beam from each beam splitter.
14. The apparatus of claim 13 wherein identical holograms are produced at each beam splitter to increase the hologram signal to noise.
15. The apparatus of claim 13 wherein holograms at different phases are produced at each beam splitter to increase hologram imaging speed.
16. An apparatus configured to produce a hologram of an object emitting electromagnetic radiation, said apparatus comprising polarization sensitive devices with characteristics similar to liquid crystal refractive or diffractive elements, wherein a path of the electromagnetic radiation within the apparatus is free of reflective optics.