IP Library › Granted Patent US 10,233,467
Granted Patent B2
US 10,233,467 · App. 14/422,180 · Granted Mar 19, 2019

Two-stage production of higher alcohols

Inventors: Yi-Xin Huo (Los Angeles, CA); Kwang Myung Cho (Sungnam-Si, KR)
Assignee: Easel Biotechnologies, LLC
C12P7/16C12M21/12C12M21/18C12M23/58C12P39/00C12Y101/01001C12Y104/01009C12P7/56C12P13/06C12P13/08Y02E50/10
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Quick Facts
Patent No.
US 10,233,467
App. No.
14/422,180
Granted
Mar 19, 2019
Kind
B2
Abstract

Methods and systems for the production of alcohols are described. A two stage process is utilized, where fermentation in a first stage produces an intermediate product, such as an amino acid or organic acid, from a carbon containing feedstock. A second stage produces alcohol by fermentation of this intermediate product.

Claims (36)

1. A method for producing branched chain or higher alcohols comprising:

providing a first bioreactor comprising a first biological catalyst system wherein the first bioreactor converts a feedstock comprising a carbon source into an amino acid product;

providing a second bioreactor comprising a second biological catalyst system;

wherein the amino acid product of the first bioreactor is introduced into the second bioreactor, and at least a portion of the amino acid product is converted in the second bioreactor into a branched chain or higher alcohol and a nitrogen byproduct,

wherein the first biological catalyst system is not capable of producing the branched chain or higher alcohol,

wherein the first biological catalyst system comprises at least one enzyme selected from the group consisting of leucine dehydrogenase (LeuDH, EC No. 1.4.1.9), threonine amino-lyase (ilvA, EC No. 4.3.1.19), acetohydroxy acid isomeroreductase (ilvC, EC No. 1.1.1.86), dihydroxy acid dehydratase (ilvD, No. 4.2.1.9), L-threonine dehydrase (tdcB, EC No. 4.3.1.17), acetolactate synthase (AlsS, EC No. 2.2.1.6), isopropylmalate synthase (LeuA, EC No. 2.3.3.13), isopropylmalate dehydrogenase (LeuB, EC No. 1.1.1.85), lactate dehydrogenase (LdhA, EC No. 1.1.1.27), EC No. 2.6.1.42, and EC number 4.2.1.33; and

wherein the second biological catalyst system comprises at least one enzyme selected from the group consisting of leucine dehydrogenase (LeuDH, EC No. 1.4.1.9), alcohol dehydrogenase (NADP+) (YqhD, EC No. 1.1.1.2), alcohol dehydrogenase (AdhP, EC No. 1.1.1.1), lactate dehydrogenase (Ldh, EC No. 1.1.1.27), acetolactate synthase (AlsS, EC No. 2.2.1.6), acetohydroxy acid isomeroreductase (ilvC, No. 1.1.1.86), dihydroxy acid dehydratase (ilvD, No. 4.2.1.9), lactate dehydrogenase (LdhA, EC No. 1.1.1.27), E.C. number 1.1.1.265, E.C. number 1.4.1.2, E.C. number 1.4.1.3, E.C. number 1.4.1.4, E.C. number 1.4.1.8, E.C. number 1.4.1.20, E.C. number 4.1.1.72, E.C. number 4.1.1.74, E.C. number 4.3.1.1, E.C. number 4.3.1.17, E.C. number 4.3.1.18, E.C. number 4.3.1.19, E.C. number 4.3.1.23, E.C. number 4.3.1.24, and E.C. number 4.3.1.25.

2. The method of claim 1 , wherein the first bioreactor further converts the carbon source of the feedstock into an organic acid.

3. The method of claim 2 , wherein the organic acid is lactic acid.

4. The method of claim 1 , wherein the branched chain or higher alcohol is selected from the group consisting of isobutanol, butanol, and 2-butanol.

5. The method of claim 1 , wherein the first biological catalyst system comprises a plurality of cells.

6. The method of claim 5 , wherein at least one of the plurality of cells originates from an organism that belongs to a genus selected from the group consisting of Clostridium, Zymonomas, Escherichia, Salmonella, Rhodococcus, Pseudomonas, Bacillus, Lactobacillus, Enterococcus, Alcaligenes, Klebsiella, Paenibacillus, Arthrobacter, Corynebacterium, Brevibacterium, Pichia, Candida, Hansenula, Synechococcus, Synechocystis, Anabaena, Ralstonia, Lactococcus , and Saccharomyces.

7. The method of claim 1 , wherein the second biological catalyst system comprises a plurality of cells.

8. The method of claim 7 , wherein at least one of the plurality of cells originates from an organism that belongs to a genus selected from the group consisting of Clostridium, Zymonomas, Escherichia, Salmonella, Rhodococcus, Pseudomonas, Bacillus, Lactobacillus, Enterococcus, Alcaligenes, Klebsiella, Paenibacillus, Arthrobacter, Corynebacterium, Brevibacterium, Pichia, Candida, Hansenula, Synechococcus, Synechocystis, Anabaena, Ralstonia, Lactococcus , and Saccharomyces.

9. The method of claim 1 , wherein the first biological catalyst system, the second biological catalyst system, or both biological catalyst systems, comprise a crude enzyme preparation, a purified enzyme preparation, or a recombinant enzyme.

10. The method of claim 1 , wherein the first biological catalyst system, the second biological catalyst system, or both biological catalyst systems comprise a substantially cell free extract.

11. The method of claim 1 , wherein the first biological catalyst system, the second biological catalyst system, or both biological catalyst systems comprise a plurality of broken or permeabilized cells.

12. The method of claim 1 , wherein the first feedstock is a gaseous carbon source.

13. The method of claim 1 , wherein the enzyme in the second biological catalyst system is alcohol dehydrogenase encoded by an adhP gene from Escherichia coli.

14. The method of claim 1 , wherein the amino acid is a branched amino acid.

15. The method of claim 1 , wherein the intermediate product is transferred into the second bioreactor.

16. The method of claim 1 , wherein, of the carbon comprising the alcohol produced in the second bioreactor, at least about 70% originates from the feedstock comprising a carbon source utilized in the first bioreactor.

17. The method of claim 1 , wherein, of the carbon comprising the alcohol produced in the second bioreactor, at least about 90% originates from the feedstock utilized in the first bioreactor.

18. The method of claim 1 , wherein the carbon source is 2-ketoisovalerate and the amino acid product is L-valine.

19. The method of claim 1 , wherein the carbon source is 2-ketoisocaproate and the amino acid product is L-leucine.

20. The method of claim 1 , wherein the carbon source is 2-keto-3-methyl-valerate and the amino acid product is L-isoleucine.

21. The method of claim 1 , wherein the carbon source is glucose and the amino acid product is L-valine.

22. The method of claim 1 , wherein the amino acid product is L-valine and the alcohol is isobutanol.

23. The method of claim 1 , wherein the amino acid product is leucine and the alcohol is 3-methyl-1-butanol.

24. The method of claim 1 , wherein the amino acid product is isoleucine and the alcohol is 2-methyl-1-butanol.

25. The method of claim 1 , wherein the carbon source is 2-ketoisocaproate and the amino acid product is L-leucine.

26. The method of claim 1 , wherein the carbon source is 2-keto-3-methyl-valerate and the amino acid product is L-isoleucine.

27. The method of claim 1 , wherein the amino acid product is L-norvaline and the alcohol is n-butanol.

28. The method of claim 1 , wherein the amino acid product is leucine and the alcohol is 3-methyl-1-butanol.

29. The method of claim 1 , wherein the amino acid product is isoleucine and the alcohol is 2-methyl-1-butanol.

30. The method of claim 22 , wherein the isobutanol concentration is at least 18 g/L.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 18, 2015
From: HUO, YI-XIN; CHO, KWANG MYUNG
To: EASEL BIOTECHNOLOGIES, LLC
Reel/Frame 034976/0441 →
Continuity (2)
Provisional Application 61684669 · Aug 17, 2012
Related Publication 20150225750A1 · Aug 13, 2015
Cited By (1)
US 12,221,404