Macrocyclic peptide, method for producing same, and screening method using macrocyclic peptide library

1. A conformationally flexible macrocyclic peptide comprising a macrocyclic structure having 4 or more amino acids,

wherein at least two amino acids of the amino acids that constitute the macrocyclic structure are not adjacent to each other, are non-proteinogenic amino acids, and have a hydrophobic side chain each independently selected from the group consisting of: (i) substituted or unsubstituted, saturated or unsaturated, and linear or branched alkyl groups having 4, 5, or 6 carbon atoms, (ii) substituted or unsubstituted aryl groups, (iii) vinyl groups, (iv) polyoxypropylene groups, and (v) polysiloxane groups; and

the hydrophobic side chains interact with each other in the macrocyclic structure in a hydrophilic environment and are exposed on the exterior of the macrocyclic structure in a hydrophobic environment.

2. The macrocyclic peptide according to claim 1 , wherein the macrocyclic structure of the macrocyclic peptide has from 5 amino acids to 20 amino acids, of which two are the amino acids having a hydrophobic side chain; and the amino acids having a hydrophobic side chain are arranged substantially opposite to each other in the macrocyclic structure.

3. The macrocyclic peptide according to claim 1 , having cell membrane permeability.

4. A method of translationally synthesizing the macrocyclic peptide as claimed in claim 1 , comprising:

a step of providing a nucleic acid that encodes the macrocyclic peptide, has codons encoding two amino acids necessary for the formation of a ring and codons encoding two amino acids having a hydrophobic side chain, and has, between two codons encoding the amino acids necessary for the formation of a ring, two codons encoding the amino acids having a hydrophobic side chain; and

a step of translating the nucleic amino acid in a cell-free translation system including tRNAs aminoacylated by the two amino acids necessary for the formation of a ring and the two amino acids having a hydrophobic side chain, respectively.

5. The method according to claim 4 , wherein at least one of the tRNAs charged with the two amino acids necessary for the formation of a ring and the two amino acids having a hydrophobic side chain, respectively, is an artificial aminoacyl tRNA.

6. The method according to claim 4 , wherein the two amino acids necessary for the formation of a ring are chloroacetylated amino acid and cysteine, respectively.

7. A screening method of the macrocyclic peptide as claimed in claim 1 having binding ability to a target molecule, comprising:

a step of forming a nucleic acid library including two or more nucleic acids including a nucleic acid that encodes the macrocyclic peptide, has codons encoding two amino acids necessary for the formation of a ring and codons encoding two amino acids having a hydrophobic side chain, has two codons encoding the amino acids having a hydrophobic side chain between two codons encoding the amino acids necessary for the formation of a ring, and includes a nucleic acid encoding a random amino acid sequence in a portion other than the codons encoding the amino acids necessary for the formation of a ring and the codons encoding the amino acids having a hydrophobic side chain;

a step of translating the nucleic acid library in a cell-free translation system to obtain a macrocyclic peptide library;

a step of bringing the macrocyclic peptide library into contact with a target molecule, followed by incubation; and

a step of selecting a macrocyclic peptide bound to the target molecule.

8. The nucleic acid library as claimed in claim 7 .

9. The macrocyclic peptide library as claimed in claim 7 .

10. A method of screening the macrocyclic peptide as claimed in claim 1 having binding ability to at target molecule, comprising:

(a) a step of forming a library including two or more mRNAs including an mRNA encoding the macrocyclic peptide, having codons encoding two amino acids necessary for the formation of a ring and codons encoding two amino acids having a hydrophobic side chain, having two codons encoding the amino acids having a hydrophobic side chain between two codons encoding an amino acid necessary for the formation of a ring, and including an mRNA encoding a random amino acid sequence in a portion other than the codons encoding the amino acids necessary for the formation of a ring and the codons encoding the amino acids having a hydrophobic side chain;

(b) a step of binding directly or indirectly puromycin to the 3′ end of each of the mRNAs of the library;

(c) a step of translating the nucleic acid of the library in a cell-free translation system to obtain an mRNA-macrocyclic peptide complex library;

(d) a step of bringing the mRNA-macrocyclic peptide complex library into contact with the target molecule, followed by incubation;

(e) selecting an mRNA-macrocyclic peptide complex group bound to the target molecule, obtaining a cDNA group by a reverse transcription reaction, and then amplifying the group; and

(f) transcribing the cDNA group to obtain an mRNA library;

wherein the steps (a) to (f) are performed twice or more to enrich the macrocyclic peptide having binding ability to the target molecule.