Method for diagnosing dental demineralization processes
The present invention relates to a quantitative ex vivo method for the complementary diagnosis of the degree of dental demineralization through the use of Raman spectroscopy, which comprises quantifying the intensities and areas of a plurality of Stoke bands of the spectrum, and defining and calculating indices that result from dividing the areas of certain bands of interest. The diagnosis is determined by the proximity of the values of these indices to the values previously obtained for the normal pattern of dental mineralization established by analyzing healthy dental parts. This method provides a complementary biomedical technique for quantitative ex vivo analysis of the degree of dental demineralization on dental tissue remains extracted by medical prescription, and the information obtained will facilitate the prescription of suitable treatment to reduce or prevent said demineralization.
1. A quantitative ex vivo method for diagnosing dental demineralization processes by Raman Spectroscopy characterized by:
quantifying in the Raman spectrum obtained after irradiating the dental part with laser light, the areas of the Stokes bands associated with PO4 −3 bending of hydroxyapatite (430 cm −1 ), PO4 −3 stretching of hydroxyapatite (960 cm −1 ) and the C—H x lipid and protein group (2941 cm −1 ) and calculating the following relative indices defined for the diagnosis:
Mineralization Index bending (IMb)=band area 430 cm −1 /band area 2941 cm −1 ;
Mineralization Index stretching (IMs)=band area 960 cm −1 /band area 2941 cm −1 ;
determining the diagnosis by the proximity of the values of said indices with the values previously established as the normal pattern with respect to the mineralization of healthy dental parts, established at: 1.35-1.75 for IMb and 4.01-5.19 for IMs.
2. The quantitative ex vivo method for diagnosing dental demineralization processes by Raman Spectroscopy according to claim 1 , characterized by the fact that said method is implemented by computer.
3. The method according to claim 1 , which is used for the complementary diagnosis of demineralization processes associated with dental caries.
4. The method according to claim 1 , which is used for the complementary diagnosis of dental demineralization processes that alter the formation and calcification of the tooth.
5. The method according to claim 2 , which is used for the complementary diagnosis of demineralization processes associated with dental caries.
6. The method according to claim 2 , which is used for the complementary diagnosis of dental demineralization processes that alter the formation and calcification of the tooth.
7. The method according to claim 3 , which is used for the complementary diagnosis of dental demineralization processes that alter the formation and calcification of the tooth.
8. The method according to claim 5 , which is used for the complementary diagnosis of dental demineralization processes that alter the formation and calcification of the tooth.
9. The method according to claim 4 , wherein the dental demineralization processes that alter the formation and calcification of the tooth are selected from fluorosis and amelogenesis imperfecta (AI).
10. The method according to claim 6 , wherein the dental demineralization processes that alter the formation and calcification of the tooth are selected from fluorosis and amelogenesis imperfecta (AI).
11. The method according to claim 7 , wherein the dental demineralization processes that alter the formation and calcification of the tooth are selected from fluorosis and amelogenesis imperfecta (AI).
12. The method according to claim 8 , wherein the dental demineralization processes that alter the formation and calcification of the tooth are selected from fluorosis and amelogenesis imperfecta (AI).