Biocatalyst solvent using ionic liquid, and biocatalyst solution containing biocatalyst and said solvent
There is provided a biocatalyst solvent which is capable of dissolving a biocatalyst in a liquid state while maintaining the activity of the biocatalyst at a high concentration from a low temperature to a high temperature, and is capable of storing the biocatalyst for a long time period; and a biocatalyst solution using the biocatalyst solvent. Disclosed is a biocatalyst solvent which consists of an ionic liquid including a quaternary ammonium cation represented by the following Formula (I) and an anion: [Chemical Formula 1] N + [R a ] n [R b ] 4-n (I) wherein R a 's each independently represent a hydroxyalkyl group having one or more hydroxyl groups and an a linear or branched alkyl moiety having 1 to 10 carbon atoms, in which the alkyl moiety may contain an oxygen atom; a carboxyalkyl group having one or more carboxyl groups and a linear or branched alkyl moiety having 1 to 10 carbon atoms, in which the alkyl moiety may contain an oxygen atom; or a hydroxycarboxyalkyl group having one or more hydroxyl groups, one or more carboxyl groups and a linear or branched alkyl moiety having 1 to 10 carbon atoms, in which the alkyl moiety may contain an oxygen atom; R b 's each independently represent a hydrogen atom, or a linear or branched alkyl group having 1 to 5 carbon atoms; and n represents an integer from 1 to 4.
1. A method for stabilizing enzyme activity during storage in a solvent comprising:
preparing an enzyme solution in which an activity of the enzyme is retained, said solution comprising the solvent and the enzyme, wherein the solvent consists of an ionic liquid including a quaternary ammonium cation represented by the following Formula (I) and an anion:
[Chemical Formula 1]
N + [R a ]n[R b ]4-n (I)
wherein R a 's each independently represent a hydroxyalkyl group having one or more hydroxyl groups and a linear or branched alkyl moiety having 1 to 10 carbon atoms, the alkyl moiety optionally containing an oxygen atom; a carboxyalkyl group having one or more carboxyl groups and a linear or branched alkyl moiety having 1 to 10 carbon atoms, the alkyl moiety optionally containing an oxygen atom; or a hydroxycarboxyalkyl group having one or more hydroxyl groups, one or more carboxyl groups and a linear or branched alkyl moiety having 1 to 10 carbon atoms, the alkyl moiety optionally containing an oxygen atom; R b 's each independently represent a hydrogen atom, or a linear or branched alkyl group having 1 to 5 carbon atoms; and n represents an integer from 1 to 4;
storing the enzyme solution for a period of time after preparing; and
subjecting the stored enzyme solution to a biochemical reaction to which the enzyme has substrate specificity,
wherein the solvent enhances the retention of the enzyme activity during said storage.
2. The method according to claim 1 , wherein each R b independently represents a hydrogen atom, a methyl group or an ethyl group.
3. The method according to claim 2 , wherein each R b represents a hydrogen atom.
4. The method according to claim 2 , wherein the quaternary ammonium cation represented by Formula (I) in the ionic liquid has a structure wherein n is 1 to 3.
5. The method according to claim 3 , wherein the quaternary ammonium cation represented by Formula (I) in the ionic liquid has a structure wherein n is 1 to 3.
6. The method according to claim 1 , wherein the anion of the ionic liquid is at least one selected from the group consisting of a halogen-based anion, a sulfur-based anion, a phosphorous-based anion, a cyan-based anion, a boron-based anion, a fluorine-based anion, a nitrogen oxide-based anion and a carboxylate anion.
7. The method according to claim 6 , wherein the anion of the ionic liquid is at least one selected from the group consisting of a carboxylate anion, a sulfonate anion and a phosphate anion.