Method for cross-section processing and observation and apparatus therefor
A method for cross-section processing and observation, and apparatus therefor, includes performing a position information obtaining process of observing the entirety of a sample by using an optical microscope or an electron microscope, and obtaining three-dimensional position coordinate information of a particular observation target object included in the sample; performing a cross-section processing process of irradiating a particular region in which the object is present by using a focused ion beam based on the information, and exposing a cross section of the region; performing a cross-section image obtaining process of irradiating the cross section by using an electron beam, and obtaining a cross-section image of a predetermined size region including the object; and performing a three-dimensional image obtaining process of repeating the cross-section processing process and the cross-section image obtaining process at predetermined intervals in a predetermined direction, and obtaining a three-dimensional image from the multiple cross-section images.
1. A method for cross-section processing and observation, the method comprising:
a process of obtaining position information, by observing the entirety of a sample of observation target through a confocal stereoscopic microscope, obtaining an approximate three-dimensional position coordinate information of a particular observation target object included in the sample;
a process of cross-section processing, based on the three-dimensional position coordinate information, by irradiating a focused ion beam towards a particular region in which the particular observation target object is present in the sample, exposing a cross-section of the particular region;
a process of obtaining a cross-sectional image, by irradiating an electron beam on the cross-section, obtaining an image of a region of a predetermined size including the particular observation target object; and
a process of generating a three-dimensional image, by repeatedly performing the process of cross-section processing and the process of obtaining a cross-sectional image at predetermined intervals along a predetermined direction a plurality of times, constructing a three-dimensional image including the particular observation target object from a plurality of the cross-sectional images obtained.
2. The method of claim 1 , wherein plural kinds of the particular observation target objects are set, and the process of cross-section processing is performed on each of the particular observation target objects.
3. The method of claim 1 , wherein the process of obtaining a cross-sectional image comprises additionally obtaining a cross-sectional composite image of the particular region including the particular observation target object by detecting energy dispersive X-rays of the cross-section and the process of generating a three-dimensional image comprises constructing a three-dimensional composite image including the particular observation target object from a plurality of the cross sectional composite images obtained.
4. An apparatus for cross-section processing and observation, the apparatus comprising:
a sample stage on which a sample including a particular observation target object is placed;
a focused ion beam column that irradiates a focused ion beam on the sample;
an electron beam column that irradiates an electron beam on the sample;
an optical microscope column that optically observes the sample;
a secondary electron detector that detects secondary electrons generated from the sample or a back-scattered electron detector that detects back-scattered electrons;
an EDS detector for detecting characteristic X-rays generated from the sample; and
a control device configured to specify a location where the particular observation target object is present within the sample by observing the entirety of the sample using the optical microscope column and obtaining an approximate three-dimensional position coordinate information of the particular observation target object included in the sample, and construct a three-dimensional image of a particular region including the particular observation target object based on the three-dimensional position coordinate information and a three-dimensional composite image of the particular region.
5. A method for cross-section processing and observation, the method comprising:
a process of obtaining position information, by observing the entirety of a sample of observation target through an optical microscope or an electron microscope, obtaining an approximate three-dimensional position coordinate information of a particular observation target object included in the sample;
a process of cross-section processing, based on the three-dimensional position coordinate information, by irradiating a focused ion beam towards a particular region in which the particular observation target object is present in the sample, exposing a cross-section of the particular region;
a process of obtaining a cross-sectional image, by irradiating an electron beam on the cross-section, obtaining an image of a region of a predetermined size including the particular observation target object; and
a process of generating a three-dimensional image, by repeatedly performing the process of cross-section processing and the process of obtaining a cross-sectional image at predetermined intervals along a predetermined direction a plurality of times, constructing a three-dimensional image including the particular observation target object from a plurality of the cross-sectional images obtained,
wherein the process of obtaining a cross-sectional image comprises additionally obtaining a cross-sectional composite image of the particular region including the particular observation target object by detecting energy dispersive X-rays of the cross-section, and the process of generating a three-dimensional image comprises constructing a three-dimensional composite image including the particular observation target object from a plurality of the cross-sectional composite images obtained.
6. The method of claim 5 , wherein plural kinds of the particular observation target objects are set, and the process of cross-section processing is performed on each of the particular observation target objects.