Composition and method for delivery of BMP-2 amplifier/co-activator for enhancement of osteogenesis
A composition comprising a synthetic growth factor analogue comprising a non-growth factor heparin binding region, a linker and a sequence that binds specifically to a cell surface receptor and an osteoconductive material where the synthetic growth factor analogue is attached to and can be released from the osteoconductive material and is an amplifier/co-activator of osteoinduction.
1. A composition comprising:
a synthetic growth factor analogue having the structure
wherein Hx is 6-aminohexanoic acid, and
an osteoconductive material comprising 60-20 wt % hydroxyapatite and 80-40 wt % tricalcium phosphate;
wherein the synthetic growth factor analogue is bound to and can be released from the osteoconductive material.
2. The composition of claim 1 , wherein the osteoconductive material comprises 60 wt % hydroxyapatite and 40 wt % tricalcium phosphate.
3. The composition of claim 1 , wherein the osteoconductive material comprises 20 wt % hydroxyapatite and 80 wt % tricalcium phosphate.
4. The composition of claim 1 , wherein the osteoconductive material is formed into granules.
5. The composition of claim 1 , wherein the composition further comprises mannitol.
6. The composition of claim 1 , wherein the composition further comprises glycine.
7. The composition of claim 1 , wherein the composition further comprises mannitol and glycine.
8. The composition of claim 1 , wherein
the composition further comprises mannitol;
the osteoconductive material comprises 20 wt % hydroxyapatite and 80 wt % tricalcium phosphate; and
the osteoconductive material is formed into granules.
9. A kit comprising:
a vial containing a synthetic growth factor analogue having the structure
wherein Hx is 6-aminohexanoic acid, and
a separate container containing an osteoconductive material comprising 60-20 wt % hydroxyapatite and 80-40 wt % tricalcium phosphate.
10. The kit of claim 9 , wherein the osteoconductive material comprises 60 wt % hydroxyapatite and 40 wt % tricalcium phosphate.
11. The kit of claim 9 , wherein the osteoconductive material comprises 20 wt % hydroxyapatite and 80 wt % tricalcium phosphate.
12. The kit of claim 9 , wherein the osteoconductive material is formed into granules.
13. The kit of claim 9 , wherein the vial further contains mannitol.
14. The kit of claim 9 , wherein the vial further contains glycine.
15. The kit of claim 9 , wherein the vial further contains mannitol and glycine.
16. The kit of claim 9 , wherein
the vial further contains mannitol;
the osteoconductive material comprises 20 wt % hydroxyapatite and 80 wt % tricalcium phosphate; and
the osteoconductive material is formed into granules.