Polypeptides having lipase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having lipase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. A composition comprising an isolated polypeptide having lipase activity and having at least 94% sequence identity to the sequence of amino acids 1 to 292 of SEQ ID NO: 2, and one or more anionic surfactants,
wherein the anionic surfactants are present at a level of from 0.1 to 60 wt %,
wherein the composition is formulated at a pH from 8.0 to 11, and
wherein the composition is more effective in removing lipid stains present at a surface in comparison with an equivalent composition lacking the lipase.
2. The composition of claim 1 , wherein the isolated polypeptide comprises or consists of amino acids 1 to 292 of SEQ ID NO: 2.
3. The composition of claim 1 , wherein the composition is selected from the group consisting of a laundry cleaning composition, a dishwashing cleaning composition, a hard-surface cleaning composition and a personal care cleaning composition.
4. The composition of claim 1 , wherein the composition is in the form of a liquid, a gel, a soap bar, a powder, a granulated solid, a tablet, or any combination thereof.
5. The composition of claim 1 , wherein the composition is effective in hydrolyzing a lipid at a temperature of from about 10° C. to 90° C.
6. The composition of claim 1 , further comprising one or more enzymes selected from the group consisting of hemicellulases, peroxidases, proteases, cellulases, xylanases, lipases, phospholipases, esterases, cutinases, pectinases, mannanases, pectate lyases, keratinases, reductases, oxidases, phenoloxidases, lipoxygenases, ligninases, pullulanases, tannases, pentosanases, malanases, β-glucanases, arabinosidases, hyaluronidase, chondroitinase, laccase, amylases, and mixtures thereof.
7. The composition of claim 1 , wherein the isolated polypeptide has greater stability than Thermomyces lanuginosus lipase in an equivalent composition.
8. The composition of claim 1 , further comprising one or more divalent cations selected from the group consisting of calcium (Ca 2+ ), magnesium (Mg 2+ ) and iron-II (Fe 2+ ).
9. The composition of claim 1 , wherein the one or more anionic surfactants comprise a sulfate or sulphonate surfactant.
10. The composition of claim 1 , wherein the one or more anionic surfactants comprise an alkyl benzene sulfonate surfactant.
11. The composition of claim 1 , wherein the one or more anionic surfactants comprise alkyl polyglucoside or an alkyl alkoxylated alcohol.
12. The composition of claim 1 , wherein the composition is more effective in laundry washing detergent compared to an equivalent composition comprising Thermomyces lanuginosus lipase.
13. A composition, comprising:
an isolated polypeptide having lipase activity, wherein the isolated polypeptide consists of a polypeptide that differs from the sequence of amino acids 1 to 292 of SEQ ID NO: 2 by at least 1 amino acid but by no more than 17 amino acids;
wherein one or more anionic surfactants are present at a level of from 1 to 50 wt %, and
wherein the composition is formulated at a pH of between 8 and 10.5.