Self-assembling protein nanostructures
Synthetic nanostructures, proteins that are useful, for example, in making synthetic nanostructures, and methods for designing such synthetic nanostructures are disclosed herein.
1. An isolated nanostructure, comprising
(a) a plurality of first proteins that self-interact to form a first multimeric substructure comprising at least one axis of rotational symmetry; and
(b) a plurality of second proteins that self-interact to form a second multimeric substructure comprising at least one axis of rotational symmetry;
wherein multiple copies of the first multimeric substructure and the second multimeric substructure interact with each other at one or more symmetrically repeated, non-natural, non-covalent protein-protein interfaces that orient the first multimeric substructures and the second multimeric substructures such that their symmetry axes are aligned with symmetry axes of the same kind in a designated mathematical symmetry group;
wherein the first protein and the second protein comprise proteins selected from the following pairs of first and second proteins:
(a) T32-28A (SEQ ID NO: 11) and T32-28B (SEQ ID NO: 12);
(b) T33-09A (SEQ ID NO: 13) and T33-09B (SEQ ID NO: 14);
(c) T33-15A (SEQ ID NO: 15) and T33-15B (SEQ ID NO: 16);
(d) T33-21A (SEQ ID NO: 17) and T33-21B (SEQ ID NO: 18);
(e) T33-28A (SEQ ID NO: 19) and T33-28B (SEQ ID NO: 20);
(f) T32-28A (SEQ ID NO: 21) and T32-28B (SEQ ID NO: 22);
(g) T33-09A (SEQ ID NO: 23) and T33-09B (SEQ ID NO: 24);
(h) T33-15A (SEQ ID NO: 25) and T33-15B (SEQ ID NO: 26);
(i) T33-21A (SEQ ID NO: 27) and T33-21B (SEQ ID NO: 28);
(i) T33-28A (SEQ ID NO: 29) and T33-28B (SEQ ID NO: 30);
(k) T32-28A (SEQ ID NO: 31) and T32-28B (SEQ ID NO: 32);
(l) T33-09A (SEQ ID NO: 33) and T33-09B (SEQ ID NO: 34);
(m) T33-15A (SEQ ID NO: 35) and T33-15B (SEQ ID NO: 36);
(n) T33-21A (SEQ ID NO: 37) and T33-21B (SEQ ID NO: 38); and
(o) T33-28A (SEQ ID NO: 39) and T33-28B (SEQ ID NO: 40).
2. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T32-28A (SEQ ID NO: 11) and T32-28B (SEQ ID NO: 12).
3. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-09A (SEQ ID NO: 13) and T33-09B (SEQ ID NO: 14).
4. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-15A (SEQ ID NO: 15) and T33-15B (SEQ ID NO: 16).
5. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-21A (SEQ ID NO: 17) and T33-21B (SEQ ID NO: 18).
6. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-28A (SEQ ID NO: 19) and T33-28B (SEQ ID NO: 20).
7. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T32-28A (SEQ ID NO: 21) and T32-28B (SEQ ID NO: 22).
8. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-09A (SEQ ID NO: 23) and T33-09B (SEQ ID NO: 24).
9. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-15A (SEQ ID NO: 25) and T33-15B (SEQ ID NO: 26).
10. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-21A (SEQ ID NO: 27) and T33-21B (SEQ ID NO: 28).
11. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-28A (SEQ ID NO: 29) and T33-28B (SEQ ID NO: 30).
12. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T32-28A (SEQ ID NO: 31) and T32-28B (SEQ ID NO: 32).
13. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-09A (SEQ ID NO: 33) and T33-09B (SEQ ID NO: 34).
14. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-15A (SEQ ID NO: 35) and T33-15B (SEQ ID NO: 36).
15. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-21A (SEQ ID NO: 37) and T33-21B (SEQ ID NO: 38).
16. The isolated nanostructure of claim 1 , wherein the first protein and the second protein comprise T33-28A (SEQ ID NO: 39) and T33-28B (SEQ ID NO: 40).
17. An isolated nanostructure, comprising
(a) a plurality of first proteins that self-interact to form a first multimeric substructure comprising at least one axis of rotational symmetry; and
(b) a plurality of second proteins that self-interact to form a second multimeric substructure comprising at least one axis of rotational symmetry;
wherein multiple copies of the first multimeric substructure and the second multimeric substructure interact with each other at one or more symmetrically repeated, non-natural, non-covalent protein-protein interferences that orient the first multimeric substructures and the second multimeric substructures such that their symmetry axes are aligned with symmetry axes of the same kind in a designated mathematical symmetry group;
wherein the first protein and the second protein comprise proteins selected from the following pairs of first and second proteins:
(a) SEQ ID NOs: 1 and 2;
(b) SEQ ID NOs: 3 and 4;
(c) SEQ ID NOs: 5 and 6;
(d) SEQ ID NOs: 7 and 8; and
(e) SEQ ID NOs: 9 and 10.
18. The isolated nanostructure of claim 17 , wherein the first protein and the second protein comprise SEQ ID NOS: 1-2.
19. The isolated nanostructure of claim 17 , wherein the first protein and the second protein comprise SEQ ID NOS: 3-4.
20. The isolated nanostructure of claim 17 , wherein the first protein and the second protein comprise SEQ ID NOS: 5-6.
21. The isolated nanostructure of claim 17 , wherein the first protein and the second protein comprise SEQ ID NOS: 7-8.
22. The isolated nanostructure of claim 17 , wherein the first protein and the second protein comprise SEQ ID NOS: 9-10.