WT1 antigenic polypeptide, and anti-tumor agent containing said polypeptide
[Problem] To provide a polypeptide which enables stimulation of numerous T cells of different derivation, and to induce strong immunological response against numerous types of cancers. [Solution] Provided is a fused polypeptide that includes a helper epitope derived from the WT1 protein, and a killer epitope derived from the WT1 protein, wherein the polypeptide includes a total of 3 to 6 of the helper epitopes and the killer epitopes per molecule of the fused polypeptide.
1. A polypeptide consisting of the amino acid sequence of SEQ ID NO: 47 or 48.
2. The polypeptide according to claim 1 , wherein the polypeptide consists of the amino acid sequence of SEQ ID NO: 47.
3. The polypeptide according to claim 1 , wherein the polypeptide consists of the amino acid sequence of SEQ ID NO: 48.
4. An anti-tumor agent comprising the polypeptide according to claim 1 as an active ingredient.
5. The anti-tumor agent according to claim 4 , further comprising antigen-presenting cells.
6. A method for in vitro producing T cells that produce a cytokine against tumor cells expressing a WT1 protein, comprising (a) incubating in vitro the polypeptide according to claim 1 with mononuclear cells, and (b) incubating the mononuclear cells from step (a) with antigen presenting cells.