Compositions and methods related to proteins capable of reversible transition to a melt
Provided are compositions and methods for making a variety of products. The methods involve mixing sucker ring teeth (SRT) protein and a plasticizer or a solvent to obtain a mixture of the SRT protein and the plasticizer. When the SRT is mixed with a plasticizer it is heated to between 32° C. and 195° C. to obtain an SRT protein melt. The melt is used to form a wide variety of products. When the SRT is mixed with a solvent, such as an organic solvent or an aqueous solvent, a solution of the SRT protein is formed, and is subsequently used to forming a product from the solution, wherein the product contains SRT protein.
1. A method of making an amorphous product, the method comprising mixing more than one distinct squid ring teeth (SRT) protein, wherein the more than one distinct SRT proteins differ from one another in amino acid sequences and wherein one of SRT protein comprises the amino acid sequence selected from the group consisting of SEQ ID NOs:1, 2, 12, 13, 14, 15, 16 and 17, and a plasticizer to obtain a mixture of the SRT proteins and the plasticizer, heating the mixture to between 32° C. and 195° C. to obtain an SRT protein melt, thereby forming the amorphous product from the SRT protein melt.
2. The method of claim 1 , wherein prior to the mixing, the SRT proteins are expressed in an in vitro cell culture by an expression vector and then separated from the cell culture.
3. The method of claim 1 comprising sonicating the mixture during or subsequent to the heating.
4. The method of claim 1 , wherein the forming the product comprises extruding the SRT protein melt, coating the SRT protein melt onto a surface, molding the SRT protein melt, or a combination thereof.
5. The method of claim 4 , wherein the method comprises heating the mixture to between 32° C. and 195° C. at least two times prior to the cooling the SRT protein melt.
6. The method of claim 1 , wherein the forming the product comprises cooling the SRT protein melt to form at least one structure having a three dimensional shape.
7. The method of claim 1 , wherein the forming the product comprises forming an adhesive and cohesive layer.
8. The method of claim 1 , wherein the forming the product comprises forming a film, fiber, ribbon or tube.
9. The method of claim 8 , wherein said forming the film comprises placing the SRT protein melt on a patterned surface.
10. The method of claim 1 , wherein the method further comprises adding a carbohydrate to the mixture of the SRT proteins and the plasticizer prior to or during the heating.