Polypeptides having catalase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having catalase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. A process for degrading or hydrolyzing a cellulosic material, comprising: contacting the cellulosic material with a cellulolytic enzyme composition and an isolated polypeptide to thereby degrade or hydrolyze the cellulosic material, wherein said isolated polypeptide has catalase activity and is selected from the group consisting of:
(a) a polypeptide comprising an amino acid sequence having at least 90% sequence identity to the amino acid sequence of amino acids 21 to 730 of SEQ ID NO: 4, and
(b) a fragment of the polypeptide of (a), wherein the fragment has catalase activity.
2. The process of claim 1 , wherein said isolated polypeptide comprises or consists of the amino acid sequence of SEQ ID NO: 4.
3. The process of claim 1 , wherein said isolated polypeptide comprises an amino acid sequence having at least 95% sequence identity to the amino acid sequence of amino acids 21 to 730 of SEQ ID NO: 4.
4. The process of claim 1 , wherein said isolated polypeptide comprises an amino acid sequence having at least 97% sequence identity to the amino acid sequence of amino acids 21 to 730 of SEQ ID NO: 4.
5. The process of claim 1 , wherein said isolated polypeptide comprises an amino acid sequence having at least 99% sequence identity to the amino acid sequence of amino acids 21 to 730 of SEQ ID NO: 4.
6. The process of claim 1 , wherein said isolated polypeptide comprises the amino acid sequence of amino acids 21 to 730 of SEQ ID NO: 4.
7. The process of claim 1 , wherein said isolated polypeptide comprises a catalytic domain, wherein the catalytic domain has catalase activity and wherein the amino acid sequence of the catalytic domain has at least 95% sequence identity to the amino acid sequence of amino acids 38 to 723 of SEQ ID NO: 4.
8. A process for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material with a cellulolytic enzyme composition and an isolated polypeptide to produce a saccharified cellulosic material, wherein said isolated polypeptide has catalase activity and is selected from the group consisting of:
(i) a polypeptide comprising an amino acid sequence having at least 90% sequence identity to the amino acid sequence of amino acids 21 to 730 of SEQ ID NO: 4, and
(ii) a fragment of the polypeptide of (i), wherein the fragment has catalase activity;
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and optionally
(c) recovering the fermentation product from the fermentation.
9. The process of claim 8 , wherein said isolated polypeptide comprises or consists of the amino acid sequence of SEQ ID NO: 4.
10. The process of claim 8 , wherein said isolated polypeptide comprises an amino acid sequence having at least 95% sequence identity to the amino acid sequence of amino acids 21 to 730 of SEQ ID NO: 4.
11. The process of claim 8 , wherein said isolated polypeptide comprises an amino acid sequence having at least 97% sequence identity to the amino acid sequence of amino acids 21 to 730 of SEQ ID NO: 4.
12. The process of claim 8 , wherein said isolated polypeptide comprises an amino acid sequence having at least 99% sequence identity to the amino acid sequence of amino acids 21 to 730 of SEQ ID NO: 4.
13. The process of claim 8 , wherein said isolated polypeptide comprises the amino acid sequence of amino acids 21 to 730 of SEQ ID NO: 4.
14. The process of claim 8 , wherein said isolated polypeptide comprises a catalytic domain, wherein the catalytic domain has catalase activity and wherein the amino acid sequence of the catalytic domain has at least 95% sequence identity to the amino acid sequence of amino acids 38 to 723 of SEQ ID NO: 4.