Non-pathogenic F18
There is provided an isolated E. coli strain deposited at the International Depositary Authority of Canada (IDAC) on Jun. 20, 2013 and attributed accession number 200613-01. There is also provided methods of using this strain for preventing edema disease or diarrhea caused by an F18 pathogenic E. coli infection in an animal.
1. A composition comprising a mixture of live Escherichia coli strains including an F4-expressing strain and an F18-expressing strain, the mixture being capable of reducing F18 intestine colonization in an animal upon intestinal delivery of the mixture more effectively relative to a comparison composition that is identical to said composition except for not including said F4-expressing strain, wherein the F18-expressing strain is the strain deposited at the International Depositary Authority of Canada (IDAC) on Jun. 20, 2013, and attributed accession number 200613-01 and the F4-expressing strain is the strain deposited at the IDAC on Jan. 21, 2005, and attributed accession number IDAC 210105-01.
2. The composition according to claim 1 , wherein the F18-expressing strain is in an amount sufficient for administration to an animal of from about 5×10 6 to about 3×10 10 CFU per animal.
3. The composition according to claim 1 , wherein the F18-expressing strain is in an amount sufficient for administration to an animal of at least 5×10 8 CFU per animal.
4. The composition according to claim 1 , wherein the F18-expressing strain is in an amount sufficient for administration to an animal of at least 1×10 9 CFU per animal.
5. The composition according to claim 1 , wherein the F18-expressing strain is in an amount sufficient for administration to an animal of at least 5×10 9 CFU per animal.
6. The composition according to claim 1 , wherein the F18-expressing strain is in an amount sufficient for administration to an animal of at least 3×10 8 CFU per animal.
7. The composition according to claim 1 , the composition comprising each strain in an amount sufficient for respective administration to an animal of at least 5×10 8 CFU per animal.
8. The composition according to claim 1 , wherein the composition is formulated for delivery to the intestine of an animal.
9. The composition according to claim 1 , wherein the composition is formulated for oral administration to an animal.
10. The composition according to claim 8 , wherein the animal is a pig.
11. The composition according to claim 10 , wherein the pig is a post-weaning pig.
12. The composition according to claim 10 , wherein the pig is a pre-weaning pig.
13. The composition according to claim 9 , wherein the animal is a pig.
14. The composition according to claim 13 , wherein the pig is a post-weaning pig.
15. The composition according to claim 13 , wherein the pig is a pre-weaning pig.
16. The composition according to claim 1 , further comprising a feed acceptable carrier.
17. The composition according to claim 16 , wherein the feed acceptable carrier is a solid carrier.
18. The composition according to claim 16 , wherein the feed acceptable carrier is a liquid carrier.