Formation and control of fluidic species
This invention generally relates to systems and methods for the formation and/or control of fluidic species, and articles produced by such systems and methods. In some cases, the invention involves unique fluid channels, systems, controls, and/or restrictions, and combinations thereof. In certain embodiments, the invention allows fluidic streams (which can be continuous or discontinuous, i.e., droplets) to be formed and/or combined, at a variety of scales, including microfluidic scales. In one set of embodiments, a fluidic stream may be produced from a channel, where a cross-sectional dimension of the fluidic stream is smaller than that of the channel, for example, through the use of structural elements, other fluids, and/or applied external fields, etc. In some cases, a Taylor cone may be produced. In another set of embodiments, a fluidic stream may be manipulated in some fashion, for example, to create tubes (which may be hollow or solid), droplets, nested tubes or droplets, arrays of tubes or droplets, meshes of tubes, etc. In some cases, droplets produced using certain embodiments of the invention may be charged or substantially charged, which may allow their further manipulation, for instance, using applied external fields. Non-limiting examples of such manipulations include producing charged droplets, coalescing droplets (especially at the microscale), synchronizing droplet formation, aligning molecules within the droplet, etc. In some cases, the droplets and/or the fluidic streams may include colloids, cells, therapeutic agents, and the like.
1. A microfluidic device for producing a plurality of droplets comprising:
a first microfluidic channel for flowing a first liquid, wherein the first microfluidic channel comprises an outlet;
a second microfluidic channel for flowing a second liquid, wherein the second microfluidic channel comprises an outlet;
a third microfluidic channel for flowing the second fluid, wherein the third microfluidic channel comprises an outlet;
a fourth microfluidic channel comprising an inlet, the inlet comprising a dimensional restriction, wherein the outlet of the second microfluidic channel meets the outlet of the first microfluidic channel, the outlet of the third microfluidic channel, and the inlet of the fourth microfluidic channel at a junction and wherein the outlets of the first and second microfluidic channels are not coaxially aligned; and
electrodes operably associated with the junction and configured to provide an electric field at the junction configured with the dimensional restriction to generate droplets of the first liquid within the second liquid in the fourth microfluidic channel.
2. The microfluidic device of claim 1 , wherein the dimensional restriction is less than 50% of the width of the first or second microfluidic channel.
3. The microfluidic device of claim 1 , wherein the first microfluidic channel is substantially perpendicular to the second microfluidic channel.
4. The microfluidic device of claim 1 , wherein the first liquid is conductive.
5. The microfluidic device of claim 1 , wherein the first liquid is aqueous.
6. The microfluidic device of claim 1 , wherein the second liquid is non-conductive.
7. The microfluidic device of claim 1 , wherein the second liquid is immiscible with the first liquid.
8. The microfluidic device of claim 7 , wherein the immiscible liquid is an oil.
9. The microfluidic device of claim 1 , further comprising a source of the first liquid.
10. The microfluidic device of claim 1 , wherein the device produces a plurality of regularly-spaced, single-file droplets.
11. The microfluidic device of claim 1 , wherein the droplets flow within the fourth microfluidic channel.
12. The microfluidic device of claim 1 , wherein the first microfluidic channel is less than 100 microns wide.
13. The microfluidic device of claim 1 , wherein the second microfluidic channel is less than 100 microns wide.
14. The microfluidic device of claim 1 , wherein the first and second microfluidic channels are fabricated from silicon, glass, or polydimethylsiloxane.
15. The microfluidic device of claim 1 , wherein the electrodes comprise silver, gold, copper, carbon, platinum, copper, tungsten, tin, cadmium, nickel, or indium tin oxide.
16. The microfluidic device of claim 1 , wherein the droplets are charged.
17. The microfluidic device of claim 1 , further comprising a voltage source.