Method of enriching for pancreatic endocrine cells that express chromogranin A
The present disclosure relates to compositions and methods comprising cell surface markers for hES-derived cells, in particular, endoderm lineage cells including pancreatic endoderm-type cells, derived from hES cells.
1. A method of enriching for pancreatic endocrine cells that express chromogranin A (CHGA), the method comprising:
a) exposing an in vitro population of pancreatic cells to a ligand that binds CD200, CD318, CD57, CD142, or CD340; and
b) either i) selecting cells that bind to the ligand that binds CD200, CD318, or CD57; or
ii) selecting cells that do not bind to the ligand that binds CD142 or CD340,
thereby enriching for pancreatic endocrine cells that express CHGA.
2. The method of claim 1 , wherein said ligand comprises an antibody or binding fragment thereof that specifically binds the cell-surface marker.
3. The method of claim 1 , wherein said ligand is associated with a detectable label.
4. The method of claim 1 , wherein said ligand is associated with a magnetic particle.
5. The method of claim 1 , wherein the selecting is performed by Fluorescence Activated Cell Sorting (FACS).
6. The method of claim 1 , comprising selecting cells that bind to the ligand that binds CD318, thereby enriching for pancreatic endocrine cells that express CHGA and insulin.
7. A method of enriching for pancreatic endocrine cells that express chromogranin A (CHGA), the method comprising:
a) exposing an in vitro population of pancreatic cells to a ligand that binds CD200, CD318, or CD57; and
b) selecting cells that bind to the ligand that binds CD200, CD318, or CD57,
thereby enriching for pancreatic endocrine cells that express CHGA.
8. A method of enriching for pancreatic endocrine cells that express chromogranin A (CHGA), the method comprising:
a) exposing an in vitro population of pancreatic cells to a ligand that binds CD142, or CD340; and
b) selecting cells that do not bind to the ligand that binds CD142 or CD340,
thereby enriching for pancreatic endocrine cells that express CHGA.