Method for inactivating target transcription factor using artificial small interfering peptide and use thereof
The present invention relates to a method for targeted inactivation of transcription factor using an artificial small interfering peptide and a use thereof. According to the present invention, an artificial small interfering peptide (a-siPEP) as a truncated from of the transcription factor for regulating transcription by dimerization was produced. It was also confirmed that, as a-siPEP forms a heterodimer with a transcription factor, DNA binding and transport into a nucleus of the transcription factor are inhibited, so that inactivation of the transcription factor is achieved at protein level. The method for inhibiting transcription factor activity using a-siPEP can replace a gene knock-out method and it allows protein-level inhibition of a transcription factor. Also, it is a transcription regulation method with high precision and high efficiency that can be applied for both monocot and dicot plants.
1. A recombinant plant vector comprising a gene encoding an artificial small interfering peptide (a-siPEP), wherein the artificial small interfering peptide is a truncated form of a plant transcription factor, wherein the plant transcription factor has the amino acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3,
with a proviso that:
(i) a dimerization domain of the plant transcription factor is included in the truncated form: and
(ii) the artificial small interfering peptide comprises amino acids 82-170 of SEQ ID NO:1 when the plant transcription factor has the amino acid sequence of SEQ ID NO:1;
the artificial small interfering peptide comprises amino acids 99-188 of SEQ ID NO:2 when the plant transcription factor has the amino acid sequence of SEQ ID NO:2; and
the artificial small interfering peptide comprises amino acids 103-192 of SEQ ID NO:3 when the plant transcription factor has the amino acid sequence of SEQ ID NO:3, wherein a plant transformed with said vector exhibits a delayed flowering phenotype.
2. A plant transformed with the recombinant plant vector described claim 1 to inhibit transcription factor activity.
3. A method for inhibiting transcription factor activity of a plant comprising transforming a plant cell with the recombinant vector of claim 1 to overexpress the gene encoding the artificial small interfering peptide.
4. A method for producing a transgenic plant with inhibited transcription factor activity comprising transforming a plant cell with the recombinant vector of claim 1 to overexpress the gene encoding the artificial small interfering peptide.
5. A transgenic plant having inhibited transcription factor activity which is produced by the method described in claim 4 .
6. The transgenic plant according to claim 5 , characterized in that the plant is either a dicot plant or a monocot plant.
7. The transgenic plant according to claim 5 , characterized in that the plant is either Arabidopsis thaliana or Brachypodium distachyon.
8. A transgenic seed of the transgenic plant described in claim 5 , wherein the transgenic seed comprises said recombinant vector.
9. A composition for inhibiting transcription factor activity of a plant which comprises the recombinant plant vector of claim 1 .
10. The recombinant vector of claim 1 , wherein the plant transcription factor has the amino acid sequence of SEQ ID NO: 1, and a plant transformed with the with the recombinant vector exhibits a delayed flowering phenotype.