Analysis of colorimetric or fluorometric test assays
The present invention provides for the analysis of colorimetric or fluorometric assays by way of capturing an image of the assay on the camera ( 32 ) built into the mobile phone ( 30 ). A disposable tool ( 20 ) is provided to enable the assay to be positioned an appropriate distance from the phone camera ( 30 ). A software application on the phone ( 30 ) can then analyze the captured image to determinate a qualitative or quantitative outcome of the assay. In many examples, the test will require no modification of the phone hardware and is thus a convenient and cheap technique for analyzing an assay. In other embodiments, other disposable items such filter(s) ( 41, 42 ) and/or additional light source(s) (LED 22 ) may be provided.
1. An assay analysis tool for use with a mobile device incorporating a camera in the analysis of a colorimetric or fluorometric assay using said mobile device incorporating the camera, the tool comprising:
a mounting surface for an assay substrate;
light directing means adapted to direct light onto the assay substrate when positioned on the mounting surface; and
spacing means comprising a flexible member configured to have a length equal to an appropriate operable distance from the camera of the mobile device to the assay substrate,
the flexible member being adapted to connect the assay analysis tool to the mobile device and adapted to enable the mounting surface and hence the assay substrate to be positioned at the appropriate operable distance from the camera of the mobile device when the flexible member connects the assay analysis tool to the mobile device,
wherein the operable distance is configured such that when the mounting surface is positioned at the operable distance from the camera of the mobile device, the camera of the mobile device is able to capture an image of the mounting surface and hence the assay substrate, and
wherein the flexible member is a cable adapted to transfer power and/or data to the assay analysis tool from the mobile device and/or data from the assay analysis tool to the mobile device for analysis.
2. The assay analysis tool according to claim 1 wherein the tool is provided with retention means on the mounting surface, the retention means adapted to retain the assay substrate in a position on or relative to the mounting surface and wherein the retention means comprise any one or more of: adhesive means; or clips or tabs provided to urge the assay substrate into contact with the surface.
3. The assay analysis tool according to claim 1 wherein the light directing means comprises a mirror.
4. The analysis tool according to claim 1 wherein the light directing means comprises a substantially transparent window portion of the mounting surface.
5. The assay analysis tool according to claim 1 comprising a physical memory, wherein an analysis software application is stored in the physical memory.
6. A method of analysing a colorimetric or fluorometric assay using a mobile device incorporating a camera, the method comprising the steps of:
positioning an assay substrate at the appropriate operable distance from the camera of the mobile device using an assay analysis tool in accordance with claim 1 ; directing light on to the assay substrate; capturing an image of the assay substrate; analysing the captured image and providing an indication of an outcome of the analysis to a user based on the analysis of the captured image.
7. The method as claimed in claim 6 wherein the analysis of the captured image is carried out by an algorithm contained in a software application running on the mobile device.
8. The method as claimed in claim 6 , further including controlling the illumination of the assay substrate by activating or deactivating an integral light source in the mobile device, a light source in the tool or an auxiliary light source.