Apparatus and method for tissue biopsy
Exemplary embodiments of an apparatus can be provided for obtaining portions or samples of tissue from a target region of a biological tissue. One or more needles can be provided that have a small internal diameter, e.g., about 1 mm or less, and the needles can be configured to extract the tissue portions when the needles are inserted into and withdrawn from the tissue. Windows and/or markings can be provided on the wall of the needles to facilitate access to the sample. The needles can be provided in an enclosure, and an actuator can be provided to direct the needles into the tissue and/or withdraw them. A plurality of tissue portions having known relative locations in the target region can be obtained, and extraction of the tissue portions can be well-tolerated by the tissue as compared with conventional punch biopsies or the like.
1. A method for obtaining at least one portion of a biological tissue for analysis, comprising:
at least partially inserting at least one hollow needle having a distal end at least partially into the biological tissue, the hollow needle including a central lumen and at least one opening on a wall thereof;
withdrawing the at least one needle from the biological tissue to extract the at least one portion of tissue, wherein the distal end of the at least one hollow needle is adapted and/or structured to facilitate extraction of the at least one portion from the tissue within the central lumen when the needle is withdrawn therefrom, and wherein a diameter of the lumen is less than about 2 mm;
positioning the at least one needle and the at least one portion of tissue retained in the at least one needle in a liquid for laboratory analysis, the at least one opening on the wall of the at least one hollow needle being adapted or structured to facilitate access of the liquid to the tissue portion while the tissue portion is retained within the central lumen; and
performing laboratory analysis on the tissue portion while the tissue portion is retained in the at least one needle.
2. The method of claim 1 , wherein the diameter of the lumen is less than about 1 mm.
3. The method of claim 1 , wherein the at least one hollow needle is a plurality of needles, and wherein the plurality of needles are inserted substantially simultaneously into the biological tissue.
4. The method of claim 1 , further comprising fixing at least a portion of the at least one needle in an embedding medium after withdrawing the at least one needle from the biological tissue.
5. The apparatus of claim 1 , wherein the at least one hollow needle is formed from one of an optically transparent or translucent material.
6. The apparatus of claim 5 , wherein the at least one hollow needle is formed of one of an acrylic, a plastic, glass, or a polymer.
7. The method of claim 1 , wherein the positioning of the at least one needle and the at least one portion of tissue retained in the at least one needle in the liquid for laboratory analysis is achieved without directly handling the needle.
8. The method of claim 1 , further comprising releasing the at least one needle from a handle and dropping the at least one needle into a vial.