Butyrate-producing bacterium and use thereof
A butyrate-producing bacterium belonging to Anaerostipes hadrus is provided. The amount of butyrate produced is at least 1.5 times that of Anaerostipes hadrus YIT 10092 T (DSM 3319 T ) and is measured by thawing a frozen stock solution of the bacterial strain (a 10% (w/v) skim milk-2% sodium glutamate solution with suspended bacterial cells) (cell count: 2.0 to 5.5×10 10 cells/mL), inoculating the solution at 1% to 4 mL of a PY liquid medium supplemented with 33 mM sodium acetate and 0.5 (w/v) % glucose (PYGA medium), followed by anaerobic culture at 37° C. for 24 hours, then inoculating the culture solution at 1% to a PYGA medium, followed by anaerobic culture at 37° C. for 24 hours, then inoculating the culture solution at 1% to a PY medium containing 33 mM sodium acetate and 0.5 (w/v) % L-sorbose, followed by anaerobic culture at 37° C. for 24 hours, and then measuring the butyrate concentration.
1. A composition for a food or drink product, medicament or feed, the composition comprising
an indigestible saccharide and
a butyrate-producing bacterium belonging to Anaerostipes hadrus ( Eubacterium hadrum ), wherein
an amount of butyrate produced is at least 1.5 times that of Anaerostipes hadrus ( Eubacterium hadrum ) YIT 10092 T (DSM 3319 T ), and
the amount of butyrate produced is measured by a method comprising
thawing a frozen stock solution of a bacterial strain, in which bacterial cells are suspended at a cell count of 2.0 to 5.5×10 10 cells/mL in a 10% (w/v) skim milk-2% sodium glutamate solution,
inoculating the solution at 1% to 4 mL of a PYGA medium, which is a PY liquid medium supplemented with 33 mM sodium acetate and 0.5 (w/v) % glucose and culturing anaerobically at 37° C. for 24 hours to obtain a first culture solution,
then inoculating the first culture solution at 1% to a PYGA medium and culturing anaerobically at 37° C. for 24 hours to obtain a second culture solution,
then inoculating the second culture solution at 1% to a PY medium containing 33 mM sodium acetate and 0.5 (w/v) % L-sorbose and culturing anaerobically at 37° C. for 24 hours to obtain a third culture solution, and
then measuring the amount of butyrate in the third culture solution.
2. The composition according to claim 1 , wherein the indigestible saccharide is one or more members selected from the group consisting of isomalt, isomaltulose, D-galactitol, D-xylitol, D-sorbitol, L-sorbose, maltitol, D-mannitol, lactitol and galactooligosaccharide.
3. The composition according to claim 1 , wherein the butyrate-producing bacterium is Anaerostipes hadrus ( Eubacterium hadrum ) YIT 12354 (NITE BP-01831) or Anaerostipes hadrus ( Eubacterium hadrum ) YIT 12355 (NITE BP-01832).
4. The composition according to claim 3 , wherein the indigestible saccharide is one or more members selected from the group consisting of isomalt,
isomaltulose, D-galactitol, D-xylitol, D-sorbitol, L-sorbose, maltitol, D-mannitol, lactitol and galactooligosaccharide.