Microspectroscope
To provide a microspectroscope that can perform a wide range mapping measurement with high sensitivity, at high speed, and with high wavelength resolution. The Raman spectroscope comprises: a unit for linearly irradiating excitation light; a movable stage for a sample; an objective lens for focusing Raman light from the linear irradiation region; an incident slit provided at the imaging position of Raman light; a spectrometer for diffusing the passing light; a CCD detector for detecting Raman spectral image; and a control device for controlling the mapping measurement by synchronizing the movable stage and the CCD detector. The control device controls the movable stage to move in the direction orthogonal to the longitudinal direction of the linear irradiation light and obtain one average spectrum. At the same time, the control device is configured to perform the cycle of the CCD detector while the stage is moving to obtain one average spectrum of the moving region of the linear irradiation region in one light detection cycle.
1. A microspectroscope for a two- or three-dimensional mapping measurement comprising:
a beam shaping unit for shaping an irradiation light into a linear shape;
a movable stage for positioning a sample at a focusing position of the linearly shaped irradiation light;
a focusing lens for focusing light from a linear irradiation region formed on the sample;
a slit that is provided at an imaging position corresponding to the linear irradiation region formed by the focusing lens and is parallel to the longitudinal direction of an image of the linear irradiation region;
a spectrometer for receiving a passing light of the slit and dispersing the image of the linear irradiation region in a direction orthogonal to the longitudinal direction of the image;
a CCD detector for detecting the dispersed image of the linear irradiation region; and
a control computer for performing the mapping measurement by synchronizing the movable stage and the CCD detector,
wherein
the CCD detector is consisted of a pixel group arranged in two directions, the longitudinal direction of the image of the linear irradiation region and a dispersing direction which is orthogonal thereto, and
the control device comprises:
a continuous scanning control program for continuously moving the linear irradiation region in a direction orthogonal to the longitudinal direction of the linear irradiation region by the movement of the movable stage without stopping in the sample;
a light detection control program for making the CCD detector perform a light detection cycle that is consisted of an exposure period and a read-out period of the CCD detector during the continuous movement of the linear irradiation region and obtaining one average spectrum of a moving range of the linear irradiation region during one light detection cycle; and
a mapping data configuration program for storing the average spectrum per each light detection cycle and configuring mapping data.
2. A microspectroscope according to claim 1 , wherein the control computer changes the moving velocity of the movable stage or the cycle period of the light detection cycle so that the moving range of the linear irradiation region during one light detection cycle moves parallel with respect to the pixel group and corresponds to a square having a length in the longitudinal direction of the linear irradiation region as one side.
3. A microspectroscope according to claim 2 , wherein the light detection control program performs a process for reading out all pixels in the pixel group collectively that are aligned in a line in the longitudinal direction of the dispersed image of the linear irradiation region in the read-out period.
4. A microspectroscope according to claim 1 , wherein the control computer changes the moving velocity of the movable stage or the cycle period of the light detection cycle so that the moving range of the linear irradiation region during one light detection cycle moves parallel with the pixel group and corresponds to a rectangle having a length in the longitudinal direction of the linear irradiation region as a long side, and the long side corresponds to n-times the short side of the rectangle, wherein n is an integer of two or more.
5. A microspectroscope according to claim 4 , wherein the light detection control program divides all pixels in the pixel group that are aligned in a line in the longitudinal direction of the dispersed image of the linear irradiation region in n groups and performs the process for collectively reading out the pixels of each group in the read-out period.
6. A microspectroscope according to claim 1 comprising:
a switch for switching the beam shaping unit to an online and offline position relative to an optical axis of the irradiation light, wherein,
when a spot diameter of which the irradiation light having a circular cross section is focused onto the sample without passing through the beam shaping unit is regarded as a focusing limit, the shape of a linear irradiation region formed on the sample through the beam shaping unit has a width dimension corresponding to the focusing limit and a length dimension that is 2 to 500 times the width dimension.
7. A microspectroscope according to claim 2 comprising:
a switch for switching the beam shaping unit to an online and offline position relative to an optical axis of the irradiation light, wherein,
when a spot diameter of which the irradiation light having a circular cross section is focused onto the sample without passing through the beam shaping unit is regarded as a focusing limit, the shape of a linear irradiation region formed on the sample through the beam shaping unit has a width dimension corresponding to the focusing limit and a length dimension that is 2 to 500 times the width dimension.
8. A microspectroscope according to claim 3 comprising:
a switch for switching the beam shaping unit to an online and offline position relative to an optical axis of the irradiation light, wherein,
when a spot diameter of which the irradiation light having a circular cross section is focused onto the sample without passing through the beam shaping unit is regarded as a focusing limit, the shape of a linear irradiation region formed on the sample through the beam shaping unit has a width dimension corresponding to the focusing limit and a length dimension that is 2 to 500 times the width dimension.
9. A microspectroscope according to claim 4 comprising:
a switch for switching the beam shaping unit to an online and offline position relative to an optical axis of the irradiation light, wherein,
when a spot diameter of which the irradiation light having a circular cross section is focused onto the sample without passing through the beam shaping unit is regarded as a focusing limit, the shape of a linear irradiation region formed on the sample through the beam shaping unit has a width dimension corresponding to the focusing limit and a length dimension that is 2 to 500 times the width dimension.
10. A microspectroscope according to claim 5 comprising:
a switch for switching the beam shaping unit to an online and offline position relative to an optical axis of the irradiation light, wherein,
when a spot diameter of which the irradiation light having a circular cross section is focused onto the sample without passing through the beam shaping unit is regarded as a focusing limit, the shape of a linear irradiation region formed on the sample through the beam shaping unit has a width dimension corresponding to the focusing limit and a length dimension that is 2 to 500 times the width dimension.