Carrier-antibody compositions and methods of making and using the same
Described herein are compositions of antibodies and carrier proteins and methods of making and using the same, in particular, as a cancer therapeutic. Also described are lyophilized compositions of antibodies and carrier proteins and methods of making and using the same, in particular, as a cancer therapeutic.
1. A lyophilized composition comprising nanoparticle complexes, wherein the nanoparticle complexes have an outside surface and comprise:
albumin;
cetuximab antibodies, each having an epidermal growth factor receptor (EGFR)-binding portion; and
paclitaxel;
said nanoparticle complexes being lyophilized, and wherein upon reconstitution with an aqueous solution an amount of the antibodies are arranged on the outside surface of the nanoparticle complexes and the nanoparticle complexes are capable of binding to EGFR in vivo.
2. The lyophilized composition of claim 1 that is stable at about 20° C. to about 25° C. for at least 3 months.
3. The lyophilized composition of claim 1 , wherein each of the nanoparticle complexes comprises between about 400 to about 800 antibodies.
4. The lyophilized composition of claim 3 , wherein less than 0.01% of nanoparticle complexes have a size greater than 800 nm.
5. The lyophilized composition of claim 1 wherein said nanoparticle complexes have an average size of about 160 nm.
6. The lyophilized composition of claim 1 , wherein the albumin is human serum albumin.
7. The lyophilized composition of claim 1 , wherein the nanoparticle complexes have a dissociation constant between about 1×10 −11 M and about 1×10 −9 M.
8. A lyophilized composition comprising nanoparticle complexes, wherein the nanoparticle complexes have an outside surface and comprise:
albumin;
cetuximab antibodies, each having an epidermal growth factor receptor (EGFR)-binding portion; and
paclitaxel;
said nanoparticle complexes being lyophilized, and wherein upon reconstitution with an aqueous solution an amount of the antibodies are arranged on the outside surface of the nanoparticle complexes and the nanoparticle complexes are capable of binding to EGFR in vivo;
wherein the lyophilized composition does not contain a bulking agent.
9. The lyophilized composition of claim 8 that is stable at about 20° C. to about 25° C. for at least 3 months.
10. The lyophilized composition of claim 8 , wherein the nanoparticle complexes comprise an average of about 100 to about 1000 antibodies.
11. The lyophilized composition of claim 10 , wherein less than 0.01% of the nanoparticle complexes have a size greater than 800 nm.
12. The lyophilized composition of claim 8 wherein said nanoparticle complexes have an average size of about 160 nm.
13. The lyophilized composition of claim 8 , wherein the albumin is human serum albumin.
14. The lyophilized composition of claim 8 , wherein the nanoparticle complexes have a dissociation constant between about 1×10 −11 M and about 1×10 −9 M.
15. The lyophilized composition of claim 1 , wherein said complexes have an average size of less than 1 μm.
16. The lyophilized composition of claim 1 , wherein said nanoparticle complexes have an average size between about 130 nm and about 800 nm.
17. The lyophilized composition of claim 1 , wherein the ratio of albumin-paclitaxel nanoparticle to antibody is between 10:1 and 10:30.
18. The lyophilized composition of claim 1 , wherein, the ratio of albumin-paclitaxel nanoparticle to antibody is between 10:2 and 10:6.
19. The lyophilized composition of claim 1 , wherein each of the nanoparticle complexes comprises between about 100 and about 1000 antibodies.
20. The lyophilized composition of claim 1 , further comprising at least one additional cancer therapeutic agent.
21. The lyophilized composition of claim 1 , wherein the antibodies are associated with the albumin through non-covalent bonds.
22. The lyophilized composition of claim 1 , wherein the paclitaxel is associated with the albumin through non-covalent bonds.