Methods and compositions for unsilencing imprinted genes
The present invention provides methods and compositions for inducing expression of Ube3a in a cell by contacting the cell with a topoisomerase inhibitor. Particular embodiments include a method of treating a genomic imprinting disorder, such as Angelman syndrome, in a subject by administering to the subject an effective amount of a topoisomerase inhibitor.
1. A method of treating Angelman syndrome or Rett syndrome in a human subject, comprising administering to the subject an effective amount of a topoisomerase II inhibitor without a DNA methyltransferase inhibitor wherein the topoisomerase II inhibitor is selected from the group consisting of an acridine derivative, a bisdioxopiperazine derivative, a podophyllotoxin derivative and any combination thereof, thereby treating Angelman syndrome or Rett syndrome in the subject.
2. The method of claim 1 , wherein the acridine derivative is Amsacrine.
3. The method of claim 1 , wherein the bisdioxopiperazine derivative is ICRF-193, dexrazoxane (ICRF-187) or a combination thereof.
4. The method of claim 1 , wherein the podophyllotoxin derivative is etoposide.
5. The method of claim 1 , wherein the topoisomerase II inhibitor has an efficiency E max of at least 1.5 fold over control.
6. The method of claim 1 , wherein the topoisomerase II inhibitor has an efficiency E max of at least 2.5 fold over control.